Correlation Between IL-1β Induced UGRP1 Expression And Fas/FasL Mediated Apoptosis Pathway In Thyroid Cells

Objective To investigate the correlation between IL-1β induced uteroglobin-related protein1（UGRP1）expression and Fas/Fas L mediated apoptosis pathway in thyroid cells.Methods1.Rat thyroid cells（FRTL-5 cells）were cultured in vitro and stimulated with different concentrations of IL-1β（0,10,20,40 ng/ml）and the same concentration of IL-1β（40ng/ml）for different durations（0,12,24,48 h）.The expression levels of factor associated suicide（Fas）m RNA and UGRP1 m RNA were detected by real-time PCR.2.The apoptosis rates of rat thyroid cells cultured in vitro were detected by flow cytometry and compared among the control group without IL-1β and anti-Fas L antibody,IL-1β（40 ng/m L）group and IL-1β（40 ng/m L）+anti-Fas L antibody group respectively.3.Cultured rat thyroid cells in vitro,anti-Fas L antibody was used to reduce the apoptosis of thyroid cells up-regulated by IL-1β.The expression levels of Fas m RNA and UGRP1 m RNA in thyroid cells of the control group without IL-1β and anti-Fas L antibody,IL-1β（40 ng/m L）group and IL-1β（40 ng/m L）+anti-Fas L antibody group were detected by real-time PCR.Results1.The expression levels of Fas m RNA in the 20 ng/m L group（1.88±0.22）and 40 ng/ml group（2.86±0.52）were higher than that in the control group（0 ng/ml）（1.01±0.19）under the stimulation of different concentrations of IL-1β detected by real-time PCR.And the difference was statistically significant（F=19.35,P=0.000 5）.2.The expression levels of Fas m RNA in the 12 h group（1.92±0.51）,24 h group（2.38±0.09）and 48 h group（3.63±0.28）were higher than that in the control group（0 h）（1.00±0.08）under the stimulation of different durations of IL-1β detected by real-time PCR.And the difference was statistically significant（F=40.31,P<0.000 1）.3.The expression levels of UGRP1 m RNA in the 10 ng/m L group（1.74±0.27）,20ng/m L group（2.34±0.45）and 40 ng/m L group（2.94±0.22）were higher than that in the control group（0 ng/m L）（1.01±0.18）under the stimulation of different concentrations of IL-1β detected by real-time PCR.And the difference was statistically significant（F=23.11,P=0.000 3）.4.The expression levels of UGRP1 m RNA in the the 24 h group（1.85±0.17）and 48 h group（2.12±0.12）were higher than that in the control group（0 h）（1.01±0.18）under the stimulation of different durations of IL-1β detected by real-time PCR.And the difference was statistically significant（F=24.96,P=0.000 2）.5.Detected by flow cytometry,the early apoptosis rates of thyroid cells in IL-1β group were increased（7.49±1.91% VS 28.46±3.17%）compared with the control group,the difference was statistically significant（P<0.001）,and the early apoptosis rates of thyroid cells in IL-1β+anti-Fas L antibody group were decreased（28.46±3.17% VS19.20±1.75%）compared with IL-1β group,the difference was statistically significant（P<0.05）.6.Detected by real-time PCR,the expression levels of Fas m RNA（2.75±0.18 VS3.03±0.16）and UGRP1 m RNA（2.22±0.31 VS 2.66±0.28）in rat thyroid cells stimulated by IL-1β showed no significant difference（P>0.05）before and after anti-Fas L antibody reduced apoptosis of cells.Conclusion1.IL-1β up-regulated Fas expression in rat thyroid cells in a dose-time dependent manner.2.IL-1β up-regulated UGRP1 expression in rat thyroid cells in a dose-time dependent manner.3.IL-1β induced high expression of UGRP1 in rat thyroid cells is not associated with Fas/Fas L mediated apoptosis pathway.