Association Between The Frequency Of Peripheral Blood Senescent CD8+T Cells And Nonalcoholic Fatty Liver Disease In Type 2 Diabetes Mellitus

Objective To investigate the frequency of peripheral blood senescent CD8+T cells in type 2 diabetes mellitus(T2DM)patients and its’ association with non-alcoholic fatty liver disease(NAFLD)as well as metabolic biomarkers.Methods 52 patients with T2 DM and NAFLD(T2DM + NAFLD group),50 with T2 DM alone(T2DM group),and 40 healthy control subjects(NC group)were studied.The immunometabolic characteristics of peripheral senescent T cells were investigated.Insulin resistance index was calculated by updated HOMA2calculator(HOMA2-IR).Results Senescent CD8+T cell level was higher in the T2 DM + NAFLD group than in the T2 DM group and NC group [(25.73±12.12 vs 17.85±8.61%),(25.73±12.12 vs17.24±6.32%),all P< 0.05].The between group differences had statistical significance(P<0.05).Multivariate linear regression analysis showed that the frequency of senescent CD8+T cells was independently correlated with body mass index(BMI)(β = 0.865,P<0.05).Pearson correlation analysis showed that senescent CD8+T cell level was positively correlated with BMI、low density lipoprotein chesterol(LDL-C)、CD3+T cell level 、senescent CD4+T cell level(r = 0.253,0.204,0.879,0.206,all P<0.05).Logistic regression analysis showed that LDL-C,BMI and senescent CD8+T cell level were the independently risk factors for NAFLD with T2DM(OR =0.030,0.027,0.005,all P< 0.05).High density lipoprotein chesterol(HDL-C)was the independently protective factor for NAFLD with T2DM(OR = 0.042,P< 0.05).Conclusions Senescent CD8+T cell level is significantly increased in patients with T2 DM and NAFLD,also closely related to obesity and lipid metabolism disorders.Thus,expended senescent CD8+T cells may play an important role in the development of T2 DM and NAFLD.