| Objectives:At present LA is one of the most effective natural super drugs antioxidan. It has special ambi-sulphurs and five dimension molecular construction,and has the noticeable ability of electrophilicity and reacting with free radical. The free radical like hydroxide radical, nitrogen monoxidum radical and peroxidize Nitroso can be cleaned up by LA. And LA also can clear the material which is easy to generate free radical such as hydrogen dioxide single state oxygen, hypochloric acid. LA as a strong oxidative stress catastaltic, it has been using to treat the patients with diabetes mellitus and its complication. The antioxidation of LA is gradually catching people's attention as the free radicle and active oxygen. Till now, there is no report about LA applied in skin flap at home. In this study, we try to investigate the mechanism of LA influencing the survival of the ischemic random skin flap by intraperitoneal injection. Methods: 78 Sprague-Dawley rats, weighting about 250 g, were randomly divided into two groups, each consisting of 39 rats: experimental group and control group. All animals were anesthetized with 2.5 percent of 5-ethyl-5-isoamylbarbituric acid (40mg/kg, ntraperitoneally). Following anesthesia, all rats were placed on a flat surface with legs extended and their backs were shaved. Caudally based random pattern skin flaps were design on central back of the rats in 2×7 cm dimensions.Which was raised from the deep fascia,and then sutured back to its original place. In experimental group LA (30mg/kg) was injected to abdominal cavity one daily for 7 days after the surgical procedure. However, in control group which was injected with same dosage of normal sodium. The change of dropsy, color, elasticity, and the survival of the skin flap were observed every postoperative day. 6 rats in each group were executed every two days beginning from postoperative day 1, and 0.5×0.5 cm size tissue was made to homogenate and centrifuge, getting from the near, mid and distal segments of the skin flap. The levels of superoxide dismutase (SOD) and malonyldialdehyde (MDA) were measured from the extracted skin tissue. On postoperative day 5, 6 rats were executed in each group, the mid and distal segment tissues were made to pathological figures to investigate information of inflammatory cell infiltration. On postoperative day 7, 9 rats were executed in each group and the areas of flap necrosis were measured and percentages of flap survivals were calculated. Result: the data shows that: No1: in experimental group every equal segment on each corresponding time, the SOD level was found to be higher (p<0.05), while, the MDA levels significantly lower (p<0.05) than that in the control group. No 2: On postoperative day 5, the pathological figure of the mid and distal segment tissues suggest that the dropsy and inflammatory cell infiltration in experimental group tissue is slighter than that in control group. No3: On postoperative day 7, the mean percentages of flap survivals in experimental and control groups were 75.31±5.88 percent and 62.54±8.58 percent, respectively. That tell us experimental group flap survivals super than that in the control group. Conclusions: we concluded that LA can improve the survival of ischemic random dorsal skin flap of rat. The mechanism is may due to that LA is scavenger for free radical, can reduce lipid peroxidation, decrease and repair cellular damage, and weaken inflammatory cell infiltration in flap tissue. |
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