Study On The Effect And The Mechanism Of EGFL7 On The Angiogenesis Of Brain Tissue After Traumatic Brain Injury

Objective:The expression of EGFL7 and its related pathway proteins PI3 K and Akt in the local brain tissue of rats after traumatic brain injury was observed dynamically to explore the effect and mechanism of EGFL7 expression on the angiogenesis of brain tissue after traumatic brain injury,and to explore the possible mechanism of the protective effect of atorvastatin on brain.Methods:Animal model of traumatic brain injury made by Feeney free fall method,Twenty seven SD rats were randomly divided into sham operated control group,trauma group and atorvastatin treatment group.Three rats in each group were killed at 6h,24 h and 72 h after operation.The expression of CD34 and microvessel density were measured by immunohistochemistry,EGFL7,PI3 K,Akt m RNA and miR-126 were analyzed by real time PCR,and EGFL7,PI3 K and Akt protein were detected by Western blot.Results:1.The animal model of traumatic brain injury was established successfully.2.Immunohistochemical staining showed that the expression of CD34 and MVD in atorvastatin group increased significantly at 6h,24 h and 72 h compared with NC group(P < 0.01);3.Real time The results of PCR showed that the expression of egfl7 mrna and miR-126 in atorvastatin treated group was significantly increased and significantly decreased compared with the control group and trauma group(P < 0.05),and the correlation analysis showed that the expression of miR-126 and egfl7 mrna was negatively correlated(P < 0.01);in addition,compared with NC group,the expression of PI3 K,p-pi3 k,p-Akt m RNA in trauma group and atorvastatin group,Compared with NC group,EGFL7 protein expression in trauma group and atorvastatin group increased obvious(P< 0.05).the expression of EGFL7 protein in the atorvastatin treatment group was significantly increased(P< 0.01),and the expression of EGFL7 protein in the trauma group and the treatment group at different times was significantly different(P < 0.05).In addition,the protein expression of PI3 K,p-pi3 k and p-Akt in trauma group and atorvastatin treatment group was significantly higher than other group,the difference was obvious(P < 0.05),but there was no significant difference in Akt protein expression(P > 0.05).Conclusions:After traumatic brain injury,miR-126 may regulate the microvascular repair process of local brain tissue through EGFL7/PI3K/Akt pathway,and atorvastatin may regulate the angiogenesis through EGFL7/PI3K/Akt pathway,so as to play the role of brain tissue protection.