Effect Of ERK1/2-mediated P70S6K Signaling Pathway In Sufentanil Post-treatment On Myocardial Cells Of Rats With Ischemiareperfusion Injury

ObjectiveTo investigate the Effect of ERK1/2-mediated P70S6 K signaling pathway in sufentanil post-treatment on myocardial cells of rats with ischemiareperfusion injury.MethodsSeventy-two SD male rats were randomly divided into 6 groups: sham operation group(Sham group);ischemia-reperfusion group(I/R group);DMSO group(PD98059 solvent dimethyl sulfoxide group);Shufentai Post-treatment group(Sufen group);PD group(PD98059,an inhibitor of ERK);Sufen + PD group,except for the Sham group,all groups were ischemic for 30 minutes After reperfusion for 2h.HR and MAP were recorded immediately before ischemia(T0),reperfusion 30min(T1),reperfusion 60min(T2),reperfusion90min(T3),and 120min(T4).The extent of myocardial infarction was measured at the end of reperfusion;p-ERK1/2,p-p70S6 K,Porimin and Caspase-8 protein expressions were detected by Western blot.ResultsCompared with T0,MAP and HR decreased in I / R,Sufen,DMSO,PD,and PD + Sufen groups at T1-T4(P<0.05).Compared with Sham group,HR in other groups decreased and MAP increased(P<0.05).Compared with I / R group,HR decreased and MAP increased in Sufen group(P<0.05).Compared with I / R group(45.67 ± 10.32%),the myocardial infarction range in Sufen group(20.92 ± 6.88%).Decrease(P<0.05).Compared with Sufen group,therange of myocardial infarction in PD + Sufen group(20.14 ± 6.02)increased(P<0.05).Compared with Sham group,p-p70S6 K in I / R group,Sufen group,and DMSO group.The expressions were all up-regulated(P<0.05).Compared with the I / R group,the expressions of p-p70S6 K and p-ERK1 / 2 in the Sufen group were further up-regulated(P<0.05).Compared with the SHAM group,the Porimin protein and Caspase-8 protein expressions were all up-regulated(P<0.05);compared with the I / R group,the expressions of Porimin and Caspase-8 were down-regulated in the Sufen group(P <0.05);but there was no significant difference between the groups with the DMSO,PD,and PD +Sufen groups(P>0.05).Conclusions1.Sufentanil post-treatment can improve cardiac function indexes of rats during myocardial ischemia-reperfusion,and the scope of myocardial infarction is reduced,indicating that sufentanil post-treatment can reduce myocardial damage after ischemia-reperfusion.Myocardial ischemia-reperfusion injury in rats has a protective effect.2.Sufentanil can activate p70S6 K phosphorylation and ERK1 / 2 pathway to reduce the symptoms of MIRI,at the same time inhibit apoptosis during reperfusion,and exert its protective effect on myocardial ischemia-reperfusion injury.