Effect Of ERK1/2 Signaling Pathway On Carbamylated Erythropoietin-induced Cardio-protection Against Ischemia-reperfusion Injury

Objective To investigate the effect of extracellular signal-regulated kinase 1/2(ERK1/2) signaling pathway on carbamylated erythropoietin-induced cardioprotection against ischemia-reperfusion (I/R) injury.Methods Forty rabbits were randomly allocated to control group, I/R group, I/R+CEPO group, I/R+PD98059 group, I/R+CEPO+PD98059 group. We compare the difference of infarct size using observations of ST↑,NST↑%, NQ% in the 12-lead ECG The paraffin slices were subjected to terminal deoxy-nucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining for detection of cardiac myocyte apoptotic rate.Results There was no diference between I/R+PD98059 group and I/R group on ST↑, NST↑%, NQ% and cardiac myocyte apoptotic rate (P>0.05); I/R+CEPO+PD98059 group was higher than I/R+CEPO group on ST↑, NST↑%,NQ% and cardiac myocyte apoptotic rate (P<0.05); I/R+CEPO group and IR+PD98059+CEPO group were lower than I/R group on ST↑, NST↑%, NQ% and cardiac myocyte apoptotic rate (P<0.05).Conclusions Acute cardioprotective effect of CEPO was mediated by activation of ERK1/2 signaling pathway. ERK1/2 signaling pathway wasn't significantly actived in the absence of CEPO.