| Wheat is one of the most important food crops in China.In recent years,the demand for wheat in the domestic market has gradually changed from high yield to high quality,but there is still a certain gap between the quality of domestic wheat and the requirements of the market.Glutenin plays an important role in wheat quality.Using near isogenic lines of glutenin subunits as research materials can eliminate the influence of other factors and obtain more accurate quality evaluation results of glutenin subunits,so as to provide accurate guidance for high-quality wheat breeding.Winter wheat is the most important wheat type in China because of its wide planting area,large area and the largest total yield.The near isogenic lines of glutenin subunits constructed on the genetic background of the main winter wheat varieties in the main production areas in China can be used to study the quality effect of glutenin subunits,which can more effectively guide the breeding and production of wheat in China.Based on the previous study of high molecular weight glutenin subunits(HMW-GS)backcross under the genetic background of Xiaoyan 22,the high molecular weight glutenin subunits(HMW-GS)backcross population was identified by molecular markers,SDS-PAGE,a-page and field agronomic traits investigation,and the near isogenic lines of HMW-GS under the genetic background of Xiaoyan 22 were obtained;In the backcross high generation population of low molecular glutenin subunits(LMW-GS)under the genetic background of Xiaoyan 22,Zhoumai 22 and Zhoumai 23,the LMW-GS was identified by STS molecular markers and SDS-PAGE,and then the agronomic characters were investigated and compared in the field.Multiple pairs of LMW-GS near isogenic lines at Glu-A3 and Glu-B3 loci were obtained;BC6F1 progenies were obtained from five generations of backcross HMW-GS of three Xinjiang varieties(Xindong 22,Xindong 33 and Kendong 002).The main results are as follows:1.In this study,283 BC6F2 HMW-GS lines under the genetic background of Xiaoyan22 were used as research materials.The HMW-GS composition and other storage proteins of the lines were identified by SDS-PAGE and a-page electrophoresis methods.The main agronomic traits such as plant height,ear length,total spikelets and grains per ear were compared in the field.Finally,220 HMW-GS lines under the genetic background of Xiaoyan 22 were obtained,Other HMW-GS near isogenic lines with the same storage protein and agronomic traits.The subunits of Glu-A1 locus in the near isogenic lines were N,1 and 2*;The subunit types of Glu-B1 were 6+8,7+8,7+9,13+16,17+18 and 20;Glu-D1 contains subunits of 2+12,3+12,4+12 and 5+10,which form 21 HMW-GS combinations.These HMW-GS combinations form 35 pairs of near isogenic lines with different target subunits.2.In this study,401 BC6F3 LMW-GS lines under the genetic background of Xiaoyan22,Zhoumai 22 and Zhoumai 23 were used as research materials.The composition of LMW-GS at Glu-A3 and glu-b3 loci was detected by STS molecular markers.The genetic background consistency of storage protein of pure lines containing target LMW-GS was identified and the field agronomic characters were investigated and analyzed,Finally,278LMW-GS near isogenic lines with the same storage protein and agronomic traits were obtained except for the different composition of target LMW-GS.The number of near isogenic lines under the genetic background of Xiaoyan 22,Zhoumai 22 and Zhoumai 23 were 93,100 and 85 respectively.15 subunits were introduced into Glu-A3 and Glu-B3 loci.The types of subunits in Glu-A3 locus were Glu-A3 a,Glu-A3 b,Glu-A3 c,Glu-A3 d,Glu-A3 e,Glu-A3 f and Glu-A3g;The subunit types of Glu-B3 were Glu-B3 a,Glu-B3 b,Glu-B3 d,Glu-B3 e,Glu-B3 h,Glu-B3 i,Glu-B3 j and Glu-B3 g.Under the genetic background of Xiaoyan 22,Zhoumai 22 and Zhoumai 23,LMW-GS near isogenic lines with 13,14 and 16 subunits were constructed respectively,and 29,34 and 38 pairs of near isogenic lines with different target subunits were formed respectively under the three genetic backgrounds.3.In this study,423 HMW-GS progenies from three genetic backgrounds,Xindong22,Xindong 33 and Kendong 002,were used as materials.The above 423 materials were sown in October 2018,and the semi kernel detection and molecular marker detection of SDS-PAGE,backcross and self breeding in the field were carried out for two consecutive years.After the sixth backcross and one selfing,BC6 and BC6F1 generations were obtained respectively.The target subunits were identified before backcross and selfing,and the HMW-GS materials carrying the target subunits were screened out.In 2020,the number of BC6F1 populations with three genetic backgrounds of Xindong 22,Xindong 33 and Kendong 002 were 220,123 and 103,respectively,with a total of 446.HMW-GS materials with three genetic backgrounds contained 15 types of subunits,and Glu-A1 site contained N,1 and 2* subunits;The subunit types of Glu-B1 were 7+8,7OE+8,7+9,6+8,13+16,14+15,17+18 and 20;The subunit types of Glu-D1 were 2+12,3+12,4+12 and5+10.Through further screening and identification,near isogenic lines of Xindong 22,Xindong 33 and Kendong 002 were obtained. |
