Creation Of Near-Isogenic Line (NIL) With Wheat High Molecular Weight Glutenin Subunits And Their Effect On Quality

Genetic improvement of wheat quality is centralized in genetic transference of High molecular weight glutentin subunit (HMW-GS) at present. The research takes XiNong 2208,1718,1330 which are highly-product materials of different genetic base, good complex character and great potentialities of gracefully-product as receptor recurrent parents, takes 16 sorts of materials in and abroad which contain different HMW-GS as non- recurrent parents. And it takes the way of biochemical marker ,consecutive backcrossing and transbreeding to nurture 3 suits of near-isogenic line(NIL)of different HMW-GS and its groups of 3 receptor parents and research the laws of HMW-GS and subunits groups. Taking 3 suits of NIL of different genetic base as materials, research the quality efficacy of different HMW-GS and its groups under the same inherited background and research the relation of quality efficacy to inherited background of the same HMW-GS and its groups under different inherited background. Then definite good quality HMW-GS and its subunits groups for the improvement of wheat quality and seek the ideal genetic base for the improvement of wheat quality by genetic transference of good quality subunits. It provided theory and practical base for the improving of wheat quality. The result of the research are as follows:(1)Researching on electrophoresis of the half-seed without embryo of accelerate generation, groped a suit of HMW-GS SDS-PAGE method which adapts to half-seed analysis of wheat. The improvement compared with the method of predecessor are as follows: â‘ Alter the volume density(30%T)of gel hoarding liquid, and don't add SDS to separation glue buffer or condensation glue buffer, which effect the valid pore of gel molecular sieve directly. Then obtain the HMW-GS spectrum of higher resolution ratio by the alteration. â‘¡Alter the electric current intension and the proportion of H2O,CH3OH and CH3COOH in dyeing liquid and decolorant, which shorten the time of electrophoresis greatly. â‘¢Raise the consummate procedure of sample establishment, and grope the quality of extract liquid, time of vibration and calefaction which adapt to half-seed of wheat, which elevate the successful ratio of electrophoresis. (2)16 France materials which contain different HMW-GS were used to cross with 3 cultivars of GuanZhong,ShanXi. Different crosses and generation populations were obtained. The grains of 30 reciprocal F1, 15 F2, 30 backcrosses BC1F1,BC1F11 generations and their parents were analysed by SDS-PAGE method for HMW-GS genetic analysis. The results are as follows: â‘ The band patterns of HMW-GS were genetically controlled and they were not affected by environmental factors. There were large variations among cultivars; â‘¡All HMW-GS can be transmitted to the hybrid F1 as dominance from both parents and a significant effect of female parents was observed in reciprocal F1 hybrid; â‘¢The genes which control HMW-GS were inherited based on Mendel's laws of gene independent assortment and free combination.(3)The research takes the way of biochemical marker and consecutive backcrossing and transbreeding, takes XiNong 2208,1718,1330 which are highly-product materials as receptor recurrent parents, and takes 16 sorts of materials which contain different objective HMW-GS respectively as non-recurrent parents. In the way of backcrossing and half-seed SDS-PAGE electrophoresis analysis, select the half-seeds which contain the objective subunits and cultivate them under the aseptic condition. Then accelerate generation and backcross with highly-product receptor materials. Such cycle backcross 5 times. The heritability of highly-product receptor parent is up to 98.44%.Select one generation by selfing and segregation and then obtain the homozygote which contain different objective subunits. After apomixis, nurture respectively XiNong1718,2208,1330 3 suits of near-isogenic line which contain 21sorts of different objective subunits. Create good material for the research of HMW-GS quality efficacy, Gliadin and the further research of molecule level.