Identification Of Sheep Parentage Relationship And Association With Production Traits Used Microsatellite Markers

The purpose of this study is to use microsatellite markers to analyze the genetic diversity of Texel ×Kazakh hybrid sheep,to initially establish a parentage identification system suitable for this breed,and to use this system to identify parentage relationships in sheep;Simultaneous screening the microsat-ellite markers related to the production traits of Texel × Kazakh hybrid sheep provide molecular markers for genetic breeding and trait improvement of this breed.1.11 microsatellite markers with good amplification and high genetic polymorphism recommended by the United Nations Food and Agriculture Organization(FAO)and the International Animal Genetics Association(ISAG)were selected to analyze the genetic diversity of 134 Texel × Kazakh hybrid sheep.Genotyping was performed by capillary electrophoresis after multiple PCR amplification,and the parentage relationship of the Texel × Kazakh progressive F2 and the cross F2 was identified.The results show:Texel× Kazakh progressive F2 and cross F2 have 180 and 140 alleles,average alleles are 16.364 and 12.727;average expected heterozygosity are 0.807 and 0.831;average observed heterozygosity are 0.533 and 0.544;and the average polymorphic information content are 0.783 and 0.803,the polymorphic information content is greater than 0.5,indicating that the 11 loci are suitable for genetic analysis and individual identification of the population.Parental identification of 11 microsatellite marker sites in 70 candidate parents and 64 candidate progeny of the two populations showed that the cumulative exclusion probability when the parental genotype is unknown are 0.9995 and 0.9997;when the parental genotype is known the cumulative exclusion probability are 1.000;when the parental genotype is known the cumulative exclusion probability are 1.000.The 11 microsatellite marker sites have high identification efficiency in this population,can find real candidate parents for all progeny,and provide relatively accurate information for subsequent breeding work.2.The genetic diversity of 108 Texel × Kazakh hybrid sheep was detected using 11 microsatellite markers.Using the general linear model of SPSS 19.0 to analyze the correlation between different genotypes and production characters.The results show:The number of alleles in 108 Texel × Kazak hybrid sheep is 91,the average number of alleles is 8.273;the average expected heterozygosity is 0.815;the average observed heterozygosity is 0.428;the average polymorphic information content is 0.789.The loci related to birth weight,weaning weight,pre slaughter weight and carcass weight are AMEL,INRA006,INRA023;The sites related to chest width and depth are INRA063,OARFCB20;The loci related to carcass length and leg vertical length is CSRD247;The sites related to the leg length and ankle width are MAF214,ETH152,INRA005,MCM527;The loci related to leg length,hip width and shoulder width is INRA172.Explain that the selected 11 sites can be used as markers for improving traits.Through the above studies,11 microsatellite markers in Texel × Kazakh hybrid sheep was found to be rich in genetic polymorphisms,It is suitable for genetic analysis of population and paternity identification of individuals,all of which were related to productive traits have significant or extremely significant correlations,which can provide theoretical basis for trait improvement and breeding of Texel ×Kazakh hybrid sheep.