Identification Of Allelic Variation And Expression Of Vromindoline-2 In Avena Nuda L.

Avena nuda L.(2n=6x=42)is an annual herbaceous plant belonging to the genus Oats in the Gramineae family.It is a worldwide cultivated crop and an important food and feed.Naked oats originated in China and are the main cultivated species in our country.Vromindolines is starch-binding protein specific to the genus Avena,belonging to the SS protein family,with a hydrophobic domain composed of 4tryptophans and 10 cysteines,which can interact with amyloid lipids,This in turn affects the grains hardness.In this thesis,the jiepengyoumai was used for cloning and multi-copy sequence identification of An Vin-2 gene,and the An Vin-2 gene markers of the A,C and D genomes of Avena L.and the An Vin-2A,An Vin-2C and An Vin-2D of the grains of different developmental stages were developed.Exploring the expression patterns,and clarifying the difference An Vin-2A,An Vin-2-C,and An Vin-2D in 4Avena nuda L.,and comparing them 10 different hardness materials developed molecular markers related to kernel hardness.The main results are as follows:1.An Vin-2A,An Vin-2C,and An Vin-2D were cloned from Jiepengyoumai,and the genome markers were developed.2.A total of 14 multi-copy sequences of An Vin-2 were identified,14 sequences were mapped to chromosomes 4A,7C and 4D,respectively.3.The 14 copies of the sequence all contain a complete open reading frame of444 bp,encoding 147 amino acids,without introns;translation prediction proteins belong to the SS protein subfamily of the AAI＿LTSS protein superfamily,and they are all unstable hydrophilic proteins.The main function is outside the cell.4.The transcription level of An Vin-2C in different developmental stages shows from low to high and then decreases,An Vin-2D shows that it first decreases and then rises to a certain level and then decreases.The expression of An Vin-2A is not detected at all developmental stages.5.An Vin-2A was not amplified in mature seeds of 4 Avena nuda L.,but there was no significant difference between An Vin-2C and An Vin-2D in mature seeds of Avena nuda L.with different hardness.6.This research has developed two gene markers that can be used to specifically amplify An Vin-2C1 and An Vin-2C4.This study found that there are 5 allelic sequences in An Vin-2C4,namely An Vin-2C4-1,An Vin-2C4-2,An Vin-2C4-3,An Vin-2C4-4 and An Vin-2C4-5.