Study On The Tissue Culture Of Malus Micromalus Makino

In this thesis,the young stem segments obtained by hydroponic culture of the dormant branches of Malus micromalus Makino were used as explants,After experimental study on disinfection,inhibition of browning,initiation culture,detoxification,proliferation culture,rooting culture and transplanting seedling,To determine the method of explant disinfection and detoxification culture,Establish organizational training system.For the sea red fruit high-quality seedling production to provide technical suppor.The results are as follows:1.The best hydroponic method is annual branches of rough 0.8-1.3 cm,length 35 cm,Mar.before spring germination,the germination rate is 95%.2.The best disinfection method is75% ethanol 30 s 0.1% mercuric chloride 5 min2% sodium hypochlorite 3 min It has the best disinfection and sterilization effect,and has the highest germination rate,lower pollution rate and browning rate,The pollution rate was 16.67%,the browning rate was 16.67%,and the germination rate was 83.33%.3.The most suitable medium for primary culture is MS+1.0 mg/L6-BA+0.2mg/LNAA+2g/LPVP.4.The most suitable method for initial detoxification culture is Sterile group culture seedlings heats temperature treatment,urvival rate is 70.00%,Then debavirin was combined with the antiviral agent ribavirin,Optimal dosage is 20 μg/m L.The survival rate of tissue culture seedlings was 92.22%.5.The most suitable medium for proliferation culture is MS+2.0 mg/L6-BA+0.1mg/L NAA+0.3 mg/LGA3,the proliferation coefficient is 3.00.6.The optimum medium for induction of rooting was 1/2MS+0.8 mg/LIBA+0.1mg/LNAA,the highest rooting rate was 95.56%,he root system is thicker and no callus.7.The most suitable method for seedling transplanting is Humus: vermiculite =3:1,The highest survival rate was 95.56%;After transplanting,the concentration 1.0 mg/L salicylic acid The survival rate was the highest,reaching 85.00%.