Effects Of Zeolite And Biochar Addition On Microbial Community During Agricultural Waste Composting

In this experiment,agricultural solid waste was used for aerobic composting,and zeolite and biochar were added to explore its effect on the physical and chemical factors of compost and the microbial community in the composting matrix.The experiment carried out the following four treatments:the control group A without adding any reagent,the pile B with 5%zeolite added,the pile C with 5%biochar added,and the mixture with 5%zeolite and 5%biochar as a pile of D.Detect the changes of physical and chemical parameters such as temperature,water content,p H,WSOC,OM,GI,NH₄⁺-N,NO₃^--N during the composting process.Quantitative PCR and high-throughput sequencing were used to determine the abundance and structure of bacterial and fungal communities,respectively.Combined with the multivariate analysis method,the response of the additives to the environmental factors of the pile and the microbial community of the pile is analyzed and explained.The composting of this experiment went smoothly.The temperature of the compost is rapid,and the duration of the high temperature period meets the requirements of harmlessness.The physical,chemical and microbial indicators show that it complies with the laws of decomposed compost.The addition of zeolite and biochar will significantly change the structure and diversity of bacterial and fungal populations.Nocardiopsaceae,Bacillaceae,Leuconostocaceae,Phyllobacteriaceae,Xanthomonadaceae,Moraxellaceae,Cellvibrionaceae,Paenibacillaceae,Flavobacteriaceae,Sphingobacteriaceae,Streptosporangiaceae,Planococcaceae are the predominant bacterial groups.Trichocomaceae,norank＿Saccharomycetales,unclassified＿Saccharomycetales,Wallemiaceae,unclassified＿Sordarialesetc.are the dominant fungi in each stack.Through redundant analysis,it was found that the change of bacterial abundance was significantly related to the OM,NO₃^--N,p H,WSOC of the physical and chemical properties of the reactor（P<0.05）,the physicochemical factors that were significantly affected by the fungal population were OM,WSOC,NO₃^--N（P<0.05）,OM and NO₃^--N are the two most important factors that affect the bacterial and fungal population changes in the composting process of straw composting.The addition of biochar,zeolite and their combination has a positive effect on improving the degradation and humification of organic matter and increasing the effective nutrient content in the process of composting agricultural waste.