On Proteomics And Cathepsin L-like Cysteine Protein Of Hemolymph From H.flava

Ticks are common surface parasites that feed on the blood of their hosts,While biting the host,it can spread a variety of diseases and bring huge economic losses to public health security,Therefore,ticks have always been the focus of parasite researchers in various countries,and the control of ticks and tick-borne diseases is urgent.The aim of this study was to determine the protein components in haemolymph,Revealed the expression of Cathepsin L-like Cysteine protein in different tissues of Haemaphysalis flava,This will lay a foundation for the screening of anti-tick vaccine.In this study,Haemaphysalis flava haemolymph was collected by amputation method,its protein composition was digested by FASP,analysis and determination by LC/MS/MS and identification based on salivary transcriptome libraries.H.flava hemolymph samples were detected 805 peptides,identifying 152 Pro Seq,Among them,there are 140 highly trusted proseqs(unique peptide≥2,matched with unique peptide793 peptide).Cathepsin L-like cysteine proteine is one of the many highly trusted proteins in this appraisal,its coding sequence(Unigene)Contig731 was missing 5 ’-end,and 3’-end sequences.Primers were designed from existing sequences and the full-length sequence was obtained by RACE method.The results showed that the full length of Cathepsin l-like enzyme synthesis m RNA was 1547 bp and the open reading frame was 1125 bp.RT-q PCR analysis showed that Cathepsin L-like cysteine proteine had the highest expression in the salivary glands,followed by the body shell,and the lowest expression in the ovary of half-blood female ticks.Haemolymph is the most important body fluid in ticks and plays an important role in nutrient and gas transport.The results of this study are of great significance for understanding tick life processes,screening antigenic proteins and creating antitick vaccines.