Effects Of Fermented Feed On Semen Quality In Boar

With the application of artificial insemination technology in animal husbandry industry,the quality of boar semen has been widely concerned.The dietary nutrition is one of the important factors affecting boar semen quality.The study investigated the effects of fermented feed the blood indicators,semen quality and faecal microbiota of Landrace boars.Twenty adult Landrace boars were divided into 5 groups with 4 boars in each group.The boars in the control group were fed the basal ration of the boars,and the rations of the boars in the four experimental groups were 5%,10%,15%,and 20% of the basal ration replaced by fermented feed.The blood of the boar was collected on the 50 th day of the experiment,and the semen and feces of the boar were collected on the 60 th day of feeding.Measure blood biochemistry and immunity,semen quality and fecal microbe related indicators.Obtained the following results:1.The levels of IgA,IgG and IgG in the boar serum of the experimental group were significantly higher(P<0.05);but the difference in serum biochemical indicators is not significant;2.The use of 5%,10% and 15% fermented feed to replace the boar basal diet significantly improved the boar’s A-grade sperm ratio,sperm motility,curve speed,path speed and oscillating(P<0.05).At the same time,the proportion of grade B and grade C sperm was significantly reduced(P<0.05).In addition,the proportion of A+B grade sperm and the sperm linear velocity of the 10% and 15% fermented feed substitution groups were also significant compared with the control group.Increase(P<0.05);3.The semen quality of boars in the experimental group was improved effectively.The intact rate of acrosome in the four experimental groups was significantly increased(P< 0.01);compared with the control group,the sperm plasma membrane integrity rate of the10%,15% and 20% fermented feed replacement boars were all different Increase,the results of the 10% and 15% fermented feed substitution groups are extremely different(P<0.01),and the 20% group has a significant difference(P<0.05);4.Fermented feed increased the level of fructose in boar seminal pulp.The boar berry sugar level in the 5%,10%,and 15% fermented feed substitution group increased significantly(P<0.01),and the 20% fermented feed substitution group significantly increased(P<0.05);5.Fecal microbial sequencing results showed that there was no significant difference in fecal microbial richness between the experimental group and the control group(P>0.05);at the phylum level,the Tenericutes in the boar feces of the 10% fermented feed substitution group was significantly lower than that of the control group(P<0.05),and the replacement of the basic diet with 15% fermented feed significantly reduced the thick wall in the boar feces Firmicutes abundance,which increased the Bacteroidetes abundance(P<0.05);from the level of class,the abundance of Bacteroides in boar feces of the 10%and 15% fermented feed substitution groups was significantly higher than that of the control group(P<0.05).In addition,at the species level,the abundance of bacteroideae in boar feces of 10% and 15% fermented feed replacement groups was significantly higher than that of the control group(P<0.05);6.By feeding Clostridium butyricum to male mice,it was found that Clostridium butyricum could significantly increase testicular index,serum testosterone content,intestinal villus height and villus height / crypt depth values(P<0.05).In summary,the use of fermented feed to feed boars can improve the metabolism and immune function of the boars,effectively improve the semen quality of the boars,and optimize the composition of the intestinal flora of the boars.In addition,the results of this experiment suggest that the most suitable fermented feed replacement amount in the Landrace boar’s diet is 10% and 15%.