| Salt stress is one of the main environmental stresses that restrict plant growth and development.Sorghum is one of the five major cereal crops in the world,and soil salinization has seriously affected its output.In this study,the Sorghum variety Hei 63027 was used as the test material.The Sorghum seedlings at the three-leaf and one-heart stage were treated with Na Cl at a concentration of 150 m M,using High-throughput sequencing(RNA-seq)and lipidome detection Technology(Lipidomics),to detect the leaves of Sorghum seedlings under Na Cl stress.Analyzing the obtained data,and combining the physiological phenotype and gene expression regulation,the research results are as follows:1.In this study,transcriptome sequencing was performed on the leaves of Sorghum seedlings under Na Cl stress at 0 h,12 h,24 h,and 72 h.The results showed that under the screening criteria of |Log2FC|≥2,a total of 2925 differentially expressed genes were screened,including 1500 up-regulated differentially expressed genes and 1425 down-regulated differentially expressed genes.Among them,the "0 h VS 12 h" comparison group had the largest number of differential genes,which was 1059.Go classification and KEGG-pathway analysis were performed on the differentially expressed genes in each comparison group at 0 h,and the differentially expressed genes annotated to metabolic pathways were significantly enriched.Some transcription factors are involved in the regulation of salt-responsive genes in MYB,WRKY,NAC and b HLH.There are also many salt-responsive genes in hormones and osmotic regulation.2.Under Na Cl stress,it was found that the leaves of Sorghum seedlings were greatly affected by the stress by observing the phenotype,and the leaves gradually wilted.The content of osmotic adjustment substances and MDA content in the leaves of Sorghum seedlings increased with the extension of the stress time.The SOD and POD activities in the antioxidant system showed a trend of first increasing and then decreasing with the increase of the stress time,while the chlorophy II content increased during salt stress.Down has declined.At the same time,LOX genes closely related to MDA content were screened from the transcriptome data of this study.Most of the genes were up-regulated,while genes involved in chlorophyll synthesis and degradation and photosynthetic-related genes were down-regulated.Physiological indicators are consistent with gene expression,providing a theoretical basis for more thorough study of plant physiological changes under salt stress.3.In this study,Lipidomics was performed on the leaves of Sorghum seedlings under Na Cl stress at 0,3,7 and 14 days.Two kinds of galactolipids,six kinds of phospholipids and three kinds of lysophospholipids were detected.The molar percentage of MGDG and DGDG in Sorghum leaves was the highest.With the increase of the stress time,the molar percentage of DGDG is continuously increasing.The molar percentage of MGDG decreased with the increase of stress time.In phospholipids,the content of PG and PA decreased with the extension of stress time,while the trends of PC,PE,PI,and PS were opposite.C36 has the highest molar ratio in DGDG and MGDG molecular species,which proves that Sorghum is an 18:3 plant that relies on eukaryotic pathways to synthesize galactolipids.4.Through the combined analysis of transcriptome and lipidome data,the lipid-related genes obtained by screening the "0 h VS 72 h" transcriptome and the changes of membrane lipid metabolites detected by lipidome were used to construct the membrane Lipid metabolism regulation network.The up-regulation of multiple NPC and PLD genes under salt stress indicates that PLD-PA and NPC are the main ways to form DAG.It is also the main way to provide precursors for chloroplast prokaryotic pathway glycolipid synthesis under salt stress.Through the High-throughput sequencing,lipidomics detection technology and comprehensive analysis of physiological indicators to deeply explore the salt response patterns of Sorghum seedling leaves,unearth key salt-related differential genes,and analyze the physiological response mechanism of seedling leaves under salt stress.And construction of the regulation network of membrane lipid metabolism under salt stress.Provide a reference for the discovery of key genes related to salt stress and a theoretical basis for the cultivation of new Sorghum varieties... |
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