Dietary Lipid Levels Requirement Of Hybrid Yellow Catfish(Pelteobagrus Fulvidraco×p.vachelli)

A 12-week feeding trial was conducted to determine the dietary lipid levels requirements of hybrid yellow catfish(Pelteobagrus fulvidraco × P.vachelli)on the growth performance,hematological functions,serum biochemical parameters,hepatic antioxidant defense(liver&muscle),hepatic lipids index assays,digestive enzymes activities and immune response using pelleted diets containing six different lipid levels formulated to contain graded dietary lipid levels of 0.0%(control),3.4%,6.8%,10.2%,13.6%and 17%of dry matter.A total of 504 juveniles with an average initial body weight of 8.77±0.012 g was randomly divided into 6 groups containing 3 triplicates with 28 fish per each tank and the fish were fed each of the experimental diets.Effects of Dietary lipid levels on the growth performance,hematological assays and serum constituents of hybrid yellow catfishResults of the study showed that,Weight gained(WG)and specific growth rate(SGR)significantly increased by the dietary lipid’s levels in 3.4%,6.8%and 10.2%groups(P<0.05)and decreased significantly with high dietary lipid levels in 13.6%and 17%groups.The lowest(best)significant values representing FCR and PER were observed in the fish fed 10.2%dietary lipid levels compared to the other groups(P<0.05).VSI significantly showed similar results in all the fish groups fed dietary lipid levels compared to the control group(P<0.05).Effects of dietary lipid levels on the hepatic antioxidant enzymes in the Liver and Muscle and hepatic lipids assay of the hybrid yellow catfishSOD and CAT significantly showed similar results in the group fed 6.8%and 10.2%followed by 13.6%dietary lipid levels compared to control group(P<0.05)with the lowest values observed in 6.8%group.Glutathione peroxidase(GSH-Px)was significantly different with the lowest values in 6.8%followed by 10.2%than other groups(P<0.05).Malondialdehyde(MDA)showed significant difference in 6.8%and 10.2%dietary lipid levels compared to other groups(P<0.05).Hepatic lipids deposition in the liver and muscle indicated that,Glycogen levels was significantly similar in 6.8%and 13.6%compared to other groups(P<0.05).TC was significantly higher in 6.8%followed by 10.2%compared to other groups(P<0.05)while TG showed significant difference in 6.8%group than the other groups(P<0.05)Effects of dietary lipid levels on the digestive enzymes’ activities and the immune response of hybrid yellow catfishResults of digestive enzymes activities in the intestines indicated that,Trypsin levels decreased significantly in 6.8%(P<0.05)as dietary lipid levels increased while Amylase significantly decreased in 10.2%dietary lipid levels group.Fish fed 6.8%followed by 10.2%groups significantly showed a decreased lipase levels than the other groups(P<0.05).Immune response in the intestines in terms of Fish complement 3 significantly showed similar values in 6.8%and 10.2%dietary lipid levels compared to the control and 3.4%groups(P<0.05).Lysozyme activity of fish exposed to dietary lipid levels were significantly lower in 6.8%and 10.2%compared to other groups(P<0.05).Cortisol significantly increased high in 6.8%group than the other groups(P<0.05).