| Cordyceps militaris is known as a kind of precious traditional Chinese medicine with medicinal and nourishing functions,which can balance and adjust Yin and Yang at the same time.However,the wild resources of Cordyceps militaris are scarce and the quantity is small.Since the artificial culture of Cordyceps sinensis came into being in 1987,it has been successfully cultivated on tussah and mulberry silkworm,relatively few Cordyceps militaris were cultivated from cassava silkworm pupae.In the present study,cassava leaves were used to raise cassava silkworm pupae,inoculated Cordyceps sinensis with silkworm pupae,cultured Cordyceps militaris and silkworm insect grass.The common bioactive components of cassava silkworm chrysalis and Cordyceps sinensis were compared and analyzed.The non-targeted components of metabolomics were compared with that of rice base Cordyceps militaris.Providing new ideas for extending cassava industry chain and increasing the high value-added of cassava,and increased the added value of cassava to provide theoretical basis for the health care function of Cordyceps militaris from cassava silkworm.The main results of this experiment are as follows:1.Three different genotypes of cassava leaves were fed to cassava silkworm for18～25 days and mature pupae were harvested.A total of 100 silkworms and silkworm pupae of 5th ages fed with different genotypes of cassava leaves were weighed for multiple comparison and analysis of variance.The cassava silkworm fed with mixed different genotypes of cassava leaves is the heaviest and firstly to form a cocoon,which is significantly different from the weight of the cassava silkworm fed by SC9,mosaic cassava,and purple cassava（p（27）0.01）.However,the cassava silkworm pupae fed with SC9 leaves was the best and had the largest weight,which was not significantly different from silkworm pupae fed with mixed different genotypes of cassava leaves（p（29）0.05）,It is different from silkworm pupae fed with the leaves of mosaic and purple cassava（p（27）0.05）。2.The cassava silkworm pupae fed with different genotypes were artificially inoculated with Cordyceps sinensis under 18℃,80%humidity and weak light cultured conditions.After inoculation,silkworm pupae began to be hardened and grow hyphae.The pupae fed with SC9 leaves and mixed leaves were firstly to produce hyphae,and after 7d,to produce subsets of primordium,followed by feeding with purple cassava leaves to produce Cordyceps sinensis in 10 days.After the silkworm pupae of cassava leaves were ossification,there was basically no Cordyceps sinensis or individual less than a centimeter of cordyceps.After 35～50 days,the mature Cordyceps militaris was harvested,the sporophores was cylindrical,up to 12 cm,and the moisture content was 62.43%.Cordyceps militaris fed SC9 leaves is obviously stronger and better in shape than others.The 5th instar larvae of cassava silkworms to be inoculated cordyceps,the infection rate is slower than silkworm pupae.They begin to be ossified for 5d,10 d grew hyphae,20～25 d grow cordyceps,52～60 d harvest mature cordyceps.3.Cordyceps militaris of cassava contains a large number of active substances with health value.Sporocarp contains cordycepic acid18.24 g·（100 g）-1,Cordycepin 2.76 mg·g-1,total polysaccharide 7.33 g·（100 g）-1,inosine 2.49 mg·g-1,adenosine 1.86 mg·g-1,ergosterol 2.41 mg·g-1,and pentostatin 10.46 mg·g-1.Non-targeted metabolomics data showed:The difference of the content of acetyl isoniazid between the fruiting body of cassava Cordyceps militaris and the fruiting body of rice Cordyceps militaris was as high as 34.97.The differences of coumaric acid were 7.88,ergoxine 4.50,streptomycin 4.47,l-tyrosine 3.89,epigallocatechin 3.55,citalopram 2.89,and amperamycin 2.34,etc.The data showed that not only the fruit bodies of Cordyceps militariae had medicinal metabolites,but the pupae also contained abundant medicinal metabolites after inoculation,such as 12-hydroxydihydrobilirubin,s-lactose glutathione,iso-nicotomic acid,hetero-immunoglobulin E and so on.4.There were 184 metabolic pathways in Cordyceps militaris compared with those cultured in rice solid medium and cassava pupae.There were 67 significant differential signaling pathways and 36 significant differential metabolic pathways.The pupae inoculated with Cordyceps sinensis compared with uninoculaed samples were enriched to209 metabolic pathways,including 60 significant differential signaling pathways and 35significant differential metabolic pathways.There were 500 differential metabolites,in which 265 up-regulated and 235 down-regulated metabolites between the sporophores from cassava pupae and rice media.There were 697 differentially metabolites between pupa and unconnected pupa,in which 289 up-regulated and 408 down-regulated differentially metabolites. |
