| Neoseiulus barkeri belongs to Acari,Phytoseiidae,Neoseiulus,and is a predatory mite living in soil or the vicinity of the plant rhizosphere,and it can feed on nematodes.In this paper,the predation function and the effects of temperature and starvation time on N.barkeri fed on Aphelenchoides besseyi and Meloidogyne incognita were studied.Meanwhile three sublethal concentrations of abamectin(LC10,LC30,LC50)and the effects of abamectin on the predation function of N.barkeri was determined.The control effect of interval coordinated using of the drug-resistant N.barkeri and abamectin on the root-knot nematode in the pot was explored by applying the abamectin and releasing the drug-resistant N.barkeri in a certain period of time.The research results were as follows:1.This study confirmed that N.barkeri had the predatory ability on A.besseyi and can complete life cycle by preying on A.besseyi.There was no significant difference of development generation time between N.barkeri fed on A.besseyi and Tyrophagus putrescentiae.However,N.barkeri fed on A.besseyi had higher fecundity,intrinsic increase rate and finite increase rate,and had lower population doubling time.The results showed that A.besseyi was more suitable for the vital movement of N.barkeri feeding A.besseyi than feeding T.putrescentiae,and N.barkeri could be used as natural enemy of A.besseyi.2.The results showed that the predatory functional response of N.barkeri on A.besseyi agreed with the type Holling-II equation.More specially,the searching efficiency of N.barkeri and the prey density was inversely proportional.Some factors may cause changes in the functional response parameters,such as the starvation length of N.barkeri and the environmental temperature,but the response type did not change.The optimalpredation condition of A.besseyi was under 25℃ and 96 h starvation time.The predation capacity could be declined when the temperature was too low or too high,the starvation time was too long.It proved that N.barkeri had the preferably ability to prey on the A.besseyi and was the ideal predator natural enemies of A.besseyi.3.The results showed that three sublethal concentrations of abamectin(LC10,LC30,LC50),all could decrease the predation function of N.barkeri on M.incognita and and cause changes in the functional response parameters,but didn’t change the the response type.4.It found that N.barkeri could effectively control the nematode when the mites were released in the rhizosphere of Ipomoea aquatic inoculated with M.incognita.The results showed that interval coordinated using of the drug-resistant N.barkeri and abamectin could increase the control effect of M.incognita.This study was the first to clarified that N.barkeri could feed on A.besseyi to complete its life cycle and reproduction.It is a natural enemy of A.besseyi and had the value of being a plant nematode biocontrol resource.It was clear that M.incognita in the rhizosphere of plants could been effectively controled when releasing the certain amount of N.barkeri.Using of abamectin could reduce predation function and controlling effect of N.barkeri,but interval coordinated useing of the drug-resistant N.barkeri and abamectin could obviously increase the controlling effect of M.incognita.These results provided a scientific basis for the control of plant nematodes diseases by using N.barkeri and further exploring the cooperation of biological control and pesticide control in order to control plant nematodes diseases more effectively. |
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