Molecular Mechanism Of ZjAUX28 And ZjSAUR32 Genes Responding To Infection Of Jujube Witche’s Broom

Ziziphus jujuba Mill.is a woody plant of Ziziphus.It is the oldest and unique fruit tree germplasm in China.It has been cultivated for more than 8000 years.Xishan Jiaozao is one of the traditional famous specialty products in Anhui Province.It is rich in various nutrients and mineral elements.Its Vc content is 593.8mg/100 g,and its nutritional value and economic value are extremely high.Xishan cedar in the cultivation of juvenile disease has also been encountered,more than 30 years ago,there are experts with copper tree as anti-jujube disease of the rootstock,over the years,the fact that coins as a rootstock can really fight jujube disease So far,this combination of stalk and ear has been widely used in jujube cultivation,to a large extent,the role of prevention and cure jujube disease,while grafting tree after the growth is better,the fruit quality has improved to a certain extent.The metabolomics and transcriptomics of the three-year-old jujube grafted seedlings were compared and analyzed.The results showed that the hormone metabolism in the leaves of different combinations was significantly changed,The anvil is mainly mediated by the metabolic pathway of the auxin metabolic pathway and participates in the resistance to jujube disease.In this paper,two IAAs transcription factors were cloned and analyzed for functional analysis to verify the mechanism of IAAs gene on the antelope stem disease.In this paper,it is expected that the auxin regulates the physiological mechanism of the antipyre sinensis,the breeding and breeding of the resistant jujube cultivars or resistant rootstocks for the prevention and cure of jujube disease and the sustainable development of jujube industry.Theoretical research significance and practical application value.In this study,two IAAs genes,ZjAUX28 and ZjSAUR32,were cloned and analyzed by bioinformatics based on the results of transcriptome and metabolomics analysis.Real-time quantitative PCR was used to analyze the expression patterns of ZjAUX28 and ZjSAUR32.The recombinant plasmids pCXSN-FLAG-AUX28 and pCXSN-FLAG-SAUR32 were constructed and transformed into Arabidopsis thaliana,and several transgenic plants were identified.GFP fusion protein pCXSN-GFP-AUX28 and pCXSN-GFP-SAUR32 were constructed successfully The nuclei were mapped to the gene.The following main results were obtained:1.Two IAAs genes,ZjAUX28 and ZjSAUR32,were cloned from the leaves of the control group on June 28,2013.The results showed that the ORF frames of ZjAUX28 were composed of 723 bases,encoding 240 amino acids,ZjSAUR32 ORF box consists of 411 bases,encoding 136 amino acids.2.Sequence analysis and phylogenetic tree analysis of IAAs gene showed that the two IAAs gene sequences cloned in this experiment were highly homologous to the gene sequences published in NCBI database.3.Real-time quantitative PCR analysis of ZjAUX28 and ZjSAUR32 showed that the expression of ZjSAUR32 was the highest in June 28,2013,and the expression of ZjAUX28 was also in June 28,2013 Day copper tree inoculation group showed higher.4.The transgenic pCXSN-FLAG-AUX28 and pCXSN-FLAG-SAUR32 were successfully constructed and transformed into Arabidopsis thaliana.The primers were designed by ZjAUX28 and ZjSAUR32 genes and hygromycin gene sequences respectively.11,and successfully constructed GFP fusion protein,subcellular localization experiments,ZjAUX28 and ZjSAUR32 are located in the nucleus.