Establishment Of Oxidative Stress Model Of Mouse Testis Induced By 3-nitropropionic Acid

In human assisted reproduction,oxidative stress can cause oxidative damage to sperm or egg cells,which can hinders development and lead to infertility.So far,one of the main reasons for the decline in the reproductive capacity is the presence of polyunsaturated fatty acids in the sperm and egg cell membranes,resulting in the attack of ROS.In order to study this in vivo,we established a mouse testicular oxidative stress model using 3-NPA.Randomly,20 Kunming male mice were selected and divided into experimental and control group.Experimental group was injected with 3-NPA intraperitoneally in three different concentration(6.25 mg/kg,12.5 mg/kg and 25 mg/kg).In contrast the control group was injected with saline.The injection process was executed twice in the morning and evening,with an interval of 12h,for a total of 7 days.After the final injection,the mice were caged in gender couples,for observation of thrombosis and birth rate.Finally,the mice of each group were sacrificed after 12 hours from the last injection,The testes,seminal vesicles,thymus,spleen and epididymis indices were measured.The sperm density,viability,linear speed and other indicators were analyzed using Tongfang animal sperm automatic analyzer.Also,concentration of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)in testis tissue were measured.It was found that 3-NPA significantly reduced the thrombosis and average birth rate;however,there was no significant effect on weight gain,thymus,and spleen indices.It was also observed that,seminal vesicle gland and testicular indices,sperm density and activity were significantly reduced;however,the movement of sperm were not significantly changed.Finally,we also found that MDA content,antioxidant enzymes GSH-Px,SOD,CAT activities,gap between spermatogenic cells and their arrangement were directly proportional to the 3-NPA concentration.From these findings we can argue that 3-NPA at optimal concentration of 12.5mg/kg can be considered one of the potent agent for establishment of a mouse testicular oxidative stress model.