| Cabbage is one of the important vegetable crops.It has low requirements on growth environment and strong adaptability.It belongs to cruciferous Brassica vegetable crop.It is widely planted in China and the United States,Britain,Japan,South Korea,India and other countries and regions,and has obvious heterosis.Turnip mosaic virus has a wide distribution range.Turnip mosaic virus disease exists in various regions,mainly harming Brassica crops,and also affecting other cruciferous vegetables,resulting in a serious decline in production.Therefore,cultivating high-quality varieties with strong disease resistance is one of the main objectives of most plant breeding programs,and is also a top priority in the current cabbage breeding work.In recent years,great progress has been made in the research on the pathogenic mechanism of turnip mosaic virus,the resistance mechanism of cabbage cabbage to turnip mosaic virus,and the cultivation of related resistance materials.In this study,a hybrid combination was prepared using self-incompatibility resistant material A21 and susceptible material P02 of cabbage as parents.The F2 gene ration formed by the F1 generation selfing,and then through selfing to F3 generation and F4 generation.Using the F4 generation segregated population as a map to construct a population.By inoculating pathogens in the F4 generation population and conducting disease investigation,and then using CAPS labeled primers to amplify the population.To construct a molecular marker genetic map of anti-TuMV in Brassica oleracea L.The genetic map was used to locate and analyze the TuMV gene of the cabbage cabbage in this experiment.The main findings are as follows:(1)Artificial virus inoculation was performed at the seedling stage of the F4 generation population,and the inoculating strain was the TuMV-C7 strain.This test uses friction inoculation.Conducting disease surveys on individual plants,the ratio of disease resistance and susceptibility in 552 cabbages was 285: 267,which was close to 1: 1.Make a two-dimensional distribution map of the distribution of the disease progression of the population,analyze it,and use SPSS program to statistically analyze the disease progression data,and find that the symptom performance conforms to the normal distribution,the skewness is 0.955,and the kurtosis is-0.735.The anti-TuMV traits in cabbage were quantitatively genetic and suitable for QTL mapping.(2)Screening of 128 pairs of CAPS-labeled primers using parental and resistance gene pools,and finally obtaining 50 differential primers,primers are distributed on two chromosomes.These primers were used for PCR amplification detection of F4 generation population,and statistical data were obtained.Use Joinmap 4.0 software to analyze and construct a genetic map to obtain a linkage map containing 43 markers and 2 linkage groups.There are 24 markers on the LG1 linkage group,the total length of the linkage group is 154.3 c M,the maximum spacing between the marks is 38.5 c M,the minimum spacing is 0.01 c M,and the average spacing is 6.4 c M.There are 19 marks on the LG2 linkage group,the total length of the linkage gro up is 143.8c M,the maximum spacing between the marks is 36.3 c M,the minimum spacing is 1.3 c M,and the average spacing is 7.6 c M.The average number of molecular markers on the linkage group was 21.5,the map distance was 298.1 c M,and the average map distance was 6.9 c M.(3)Analysis using Win QTLCart2.5 software and interval mapping method.The LOD value is greater than 3 as the threshold for the presence of QTLs.Three QTLs related to TuMV-C7 were detected,and temporarily named R01,R02,R03.R01 and R02 are located on the LG1 linkage group,and R03 is located on the LG2 linkage group.The LOD value of R01 is 3.34,the additive efficiency is-0.2822,and the contribution rate is 28.94%.The LOD value of R02 is 3.05,the additive efficiency is-0.4554,and the contribution rate is 34.21%.The LOD value of R03 is 3.19,the additive efficiency is-0.3515,and the contribution rate is 38.80%. |
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