Comparative Study On Blood Biochemical Indexes And Intestinal Microflora Between High And Low Fatty Liver Layers

Fatty Liver is a common nutritional metabolic disease that occurs in laying hens at the late stage of laying.It is caused by the disorder of lipid metabolism in the body and characterized by excessive fat deposition in the liver.The laying hens with fatty liver can reduce egg laying performance and egg quality.In severe cases,it will cause liver fat degeneration,cause liver cells to rupture,and eventually induce intrahepatic bleeding or death,affecting the normal development of the laying hen industry.Based on this,the Rhode Island red layer hens were used as the material to measure the body weight,body size,blood biochemical indicators(under feeding and fasting state)and liver triglyceride content of layer laying hens under normal feeding conditions According to the liver fat content before and after 25%,the test chickens were divided into high and low fat liver groups,and the intestinal microbial composition of the high and low fat liver group were determined.The related traits were compared between two groups to establish a screening method for individuals with high liver fat,which will lay a foundation for reducing fatty liver incidence rate in laying hensThe main results are as follows1.There are significant differences in blood biochemical indicators between feeding and fasting layer hens.By comparing the blood biochemical indicators of feeding and fasting layer hens,the results showed that aspartate aminotransferase,aspartate/glutamic acid,lactate dehydrogenase,and high-density lipoprotein of the blood in fasting laying hens were significantly higher than those in the feeding group(P<0.05),while direct bilirubin,alanine aminotransferase,white ball ratio,albumin,Glucose was significantly lower than those in the feeding group(P<0.05)2.Correlation analysis between blood biochemical indexes,body size and liver lipid content in laying hens showed that there was a very significant positive correlation between blood glucose level and liver triglyceride content in feeding layer hens(P<0.01);and a positive correlation between aspartate aminotransferase level and triglyceride content in fasting laying hens(P<0.05).There was a very significant positive correlation between body oblique length and liver triglyceride content.The above two blood indexes and body oblique length can be used as predictors of fatty liver in laying hens.3.Comparison of blood biochemical indexes between layers with high and low liver fat.According to the content of triglyceride in liver,the layers were divided into high and low liver fat groups.By comparing the blood biochemical indexes of the two groups,the results showed that under fasting condition,the serum glucose level of low liver fat group was significantly higher than that of high liver fat group(P<0.05),and the level of aspartate aminotransferase was lower than that of high liver fat group(P<0.05).4.Analysis of differences in intestinal microbes between high and low liver fat layers.Using 16S rDNA technology to analyze the differences in intestinal flora.The results showed that there was no significant difference in the diversity of intestinal flora between high and low liver fat groups in laying hens under normal feeding conditions.In the low liver fat group,Sphingomonas and Phyllobacterium higher than the high liver fat group(P<0.05),Sphingomonas can synthesize sphingosine and form sphingomyelin with fatty acid derivatives,thereby reducing the body fat utilization rate.The genus Folium can use various sugars or organic acid salts as a carbon source to compete with the host for absorption and utilization of nutrients.In the high liver fat group,Rhodococcus and Pseudomonas are significantly higher than the low liver fat group(P<0.05).Both are conditional pathogens with strong infectivity.It can cause a variety of inflammation when the body’s immunity ability is low liver fat group,which may be related to the occurrence of fatty liver inflammation in laying hens.In addition,Pseudomonas also has a strong degradation of proteins and fats,which may enhance the efficiency of digestion and absorption of nutrients in the intestine,and increase the feed intake of laying hens;The results showed that there were very significant differences in the composition of intestinal microbes between high and low liver triglyceride layer hens,which affected the body’s energy absorption and fat metabolism.There were more beneficial bacteria in the low liver fat group,and there were more conditional pathogenic or harmful bacteria in the high liver fat group,which may cause an increased risk of disease in the intestinal tract of the host,and affects the function of the "intestine-liver axis".It leads to disorder of liver cell metabolism and inflammatory reaction in the liver,which may cause fatty liver in laying hens in some cases.