Study On The Application Of Broussonetia Papyrifera L.silage To Replace Corn Silage In Holstein Reserve Cow Diet

China’s dairy industry has developed rapidly,but the deficiency of high-quality roughage and the low efficiency of inferior roughage have become a bottleneck to the further development.Broussonetia papyrifera,which has high nutritional and feeding value,and contains more microelement and flavonoids,is a high-quality feed ingredient.Twenty Holstein reserve cows were used in a single-factor,completely randomized design to research the effects of using Broussonetia papyrifera silage(BPS)as a replacements for Corn silage(COS)on apparent digestibility of nutrients,plasma parameters,ruminal fermentation,and microbial communities.Experimental treatments were four percentage of BPS 0,25,50,and 75%,as a replacements for COS(T0,T25,T50,and T75,respectively).The primary testing result of this thesis ara shown below:1.The average daily dry matter intake were compared between control treatment(treatment T0)and the three experimental treatment(treatment T25,T50,T75)were significantly difference,and increased by 8.76%,10.30%,and 12.13% respectively compared with the group T0(P<0.05).2.The content of BUN in the experimental treatment showed a significant linear effect with the increase of the replacement amount of mulberry silage(P<0.05),but the difference between the T25,T50,and T75 were not significant(P>0.05),and the content of T75 treatment was 29.82% higher than that of the T0 treatment(P <0.05).The ALB content in T25 treatment was significantly higher than that in T0 group(P<0.05).3.Compared with T0 treatment,MDA content in T50 treatment was significantly decreased by 45.8%(P<0.05),however there was no significant difference betwee n T25 and T75 treatment(P>0.05).on the contrary,Adding BPS silage had no significant effect on SOD,GSH-PX,CAT and T-AOC.4.In our study obvious trend was found to Ig A content with the amount of BPS.Compared with T0 treatment,The Ig A content in T20,T50 and T75 treatments were increased by 18.18%(P<0.05),27.27%(P<0.05)and 27.27%(P<0.05).The concentration of IL-4 in the T75 treatment was significantly higher than that in the T0 group by 36.35%(P<0.05).The difference is that the serum IL-1β content in T50 group and T75 group was significantly lower than that in T0 group.5.There were no significant differences in the Shannon index,Ace index,Chaol index and Shannoneven index of the rumen fluid bacterial flora of each treatment group(P>0.05).The relative abundance of a total of 15 phylum was exceed than 0.01%.The abundance of prevotella＿1 in T25 group and T50 group was significantly higher than that of the T0 group by 44.97%(P<0.05)and 43.21%(P<0.05).6.BPS can increase the abundance of dominant bacteria in the Holstein reserve cow feces.Meanwhile The more BPS was added,the higher the abundance of Ver rucomicrobia in feces.We research shows that the dominant genera in Holstein’s cow feces were ruminococcaceae＿ucg-005,ruminococcaceae＿ucg-010 and Rikenellaceae＿RC9＿gut＿group,in addition The relative abundance of Ruminococcaceae＿UCG-005 and Rikenellaceae＿RC9＿gut＿group in T75 group was significantly lower than that in T25 group by 20.29% and 38.64%(P<0.05).