Investigation Of Underforest Fungi Resources And Domestication And Cultivation Of Wild Lentinus Edodes In Dechang County

Dechang County is located in the middle of Liangshan Yi Autonomous Prefecture.It covers an area of 2828.35 km~2and is located in the Anning River Valley.It is the eastern edge of the Kangzang Plateau in the Hengduan Mountains.The mountains and rivers are staggered,and the terrain is complex and diverse.Dechang County has a subtropical plateau monsoon climate,no hot in summer,no cold in winter,abundant rainfall,superior ecological environment,rich biological resources and abundant vegetation.The county has a forest coverage rate of 68.4%,and there are many kinds of large fungi under the forest.This study used the combination of morphological and molecular biology to identify the species of macrofungi in Dechang County,and to understand the status of large fungal resources in Dechang County,and the relationship between macrofungal species and vegetation types.A domesticated cultivation study was carried out on wild Lentinula edodes.The research results are as follows:Through the field investigation of large fungi on understory of Dechang County,a total of626 specimens of large fungal fruit bodies were collected,and 219 species were identified,6species were only identified to genus level.These 225 species belong to 95 genera,46 families,13 orders,5 classes and 2 divisions,and there are 119 edible fungi,76 kinds of medicinal bac-teria and 42 kinds of poisonous bacteria.There are 11 dominant families(≥5 species)which in clude 166 species are accounting for 73.78%of the identified species.They are Amanitaceae,R ussulace-ae,Boletaceae,Tricholomataceae,Polyporaceae,Rhizoaceae,Agaricaceae,Clavariaceae,Hydnangiaceae,Marasmiaceae,Strophariaceae.And 96 species in 8 dominant genus(≥5 species),accounting for 42.67%of the identified species.The genera are Russula,Lactarius,Amanita,La-ccaria,Ramaria,Agaricu,Boletus,Tylopiluss.The relation of the macrofungi distribution and vegetation community types showed that the macrofungal diversity was very different in 5 planting types which Yunnan pine forest(I),evergreen coniferous forest composed of Yunnan pine,Yunnan cedar,Huashan pine and fir(II),mixed coniferous and broad-leaved forest(III),evergreen broad-leaved forest(IV),chestnut forest(V).And the occurrence trend of large fungi is:type III>type I>type II>type V>type IV,It indicates that the species of large fungi are rich in plant communities with high canopy density,abundant humus and moderate soil water content,and the extremely dry or humid environment is not suitable for the growth of large fungi.The cultivation experiment of wild lentinula edodes mushrooms found that the wild lentinula edodes hyphae fastest growth at 25°C,p H 5,and the full dark conditions.The optimum carbon source is corn,and the most suitable nitrogen source is yeast powder.Magnesium sulfate has the strongest growth promoting effect on hyphae.The wild lentinula edodes hyphae can grow in the 10 culture mediums tested,the mycelial growth on the cottonseed hull and corncob medium is better,but the 10 culture media have not produced the fruiting bodies.The mechanism for the occurrence of subentities remains to be further studied.