Research Of Multiple Resistance Of Alopecurus Japonicus To Fenoxaprop-P-ethyl And Mesosulfuron-methyl

ACCase inhibitor herbicides such as fenoxaprop-P-ethyl and ALS inhibitor herbicides such as mesosulfuron-methyl are important herbicides for controlling wheat weeds in wheat fields,due to their single site of action,plus some areas A single continuous medication habit and farming system influences more and more weeds to develop resistance to the two inhibitor herbicides,and the resistance becomes more serious.In Anhui,Jiangsu,Henan and other places,there have been many reports about the occurrence of resistance in Alopecurus japonicus.The recommended dose and even several times the recommended dose can not effectively prevent Alopecurus japonicus.Previous studies in this laboratory have found that even the populations that are more resistant to ACCase and ALS inhibitors in these areas have become an urgent problem in wheat field production.In order to clarify the resistance of wheat field to Alopecurus japonicus,the resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl,and the cause of resistance formation,safer and more efficient use of herbicides and treatment of resistant weeds in production practice The embarrassment happened.In this study,22 populations from Anhui,Jiangsu,and Henan were used as materials to study the resistance mechanism of resistant Alopecurus japonicus to fenoxaprop-P-ethyl and mesosulfuron-methyl.all results showed that:(1)The resistance levels of Japanese maiden populations collected from the wheat growing areas of Anhui,Jiangsu and Henan were determined.It was found that AH-1 and AH-2 biotypes of Alopecurus japonicus against fenoxaprop-P-ethyl and mesosulfuron-methyl Sensitive;AH-5,AH-7,AH-9,AH-10,AH-11,AH-12,AH-13,AH-14,JS-1,JS-2 and HN-1 biotypes has high resistance to fenoxaprop-P-ethyl;AH-3,AH-4,AH-5,AH-6,AH-7,AH-8,AH-13,AH-14,AH-15,JS-3,JS-4 and HN-2 and other biological types of Alopecurus japonicus showed higher resistance to mesosulfuron-methyl;AH-5,AH-7,AH-13 and AH-14 four biotypes showed high resistance to both fenoxaprop-P-ethyl and mesosulfuron-methyl.It is a multi-resistant population.(2)The ACCase gene of resistant biotypes AH-5 and JS-1 was mutated from Ile into Asn at position 2041;the ACCase gene of resistant biotypes AH-7 and AH-9 was mutated from Ile into Leu at 1781;The ACCase genes of resistant organisms AH-10,JS-2 and HN-1 have a Trp mutation to Cys at position 2027.The ALS genes of resistant biotypes AH-3,AH-4 and AH-8 were mutated from Pro into Thr at position 197;the ALS genes of resistant biotypes AH-5,AH-6 and AH-7 were mutated from Trp into Arg at position 574.The multi-resistant populations AH-5 and AH-7 have mutations in both ACCase and ALS genes.(3)Treatment with cytochrome P450 oxidase inhibitor PBO and GSTs inhibitor NDB-Cl,found that resistant biotype AH-11 has a CST-based metabolic resistance to fenoxaprop-P-ethyl,JS-3 and JS-4 organisms Types of mesosulfuron-methyl have metabolic resistance based on GSTs.The resistance of eight resistant biotypes such as AH-5,AH-7,AH-9,AH-10,AH-12,JS-1 and JS-2 to fenoxaprop-P-ethyl may be related to the body of the plant.Based on the metabolic mechanism mediated by GSTs metabolic enzymes.The resistance of resistant biotype HN-1 to fenoxaprop-P-ethyl may be independent of the mechanism of metabolic reaction mediated by GSTs in the plan.The resistance of five resistant biotypes such as AH-3,AH-5,AH-6,AH-7 and AH-8 to mesosulfuron-methyl may be related to the metabolic reaction mediated by GSTs metabolizing enzymes in plants.Mechanism related.The resistance of the resistant biotype AH-4 to mesosulfuron-methyl may not be related to the mechanism of metabolic reaction mediated by GSTs metabolizing enzymes in plants.(4)5 kinds of resistant biotypes such as AH-5,AH-7,JS-1,JS-2 and HN-1,three kinds of ACCase inhibitor herbicides such as fenoxaprop-P-ethyl,Clodinafop-propargyl and Clethodim Cross-resistance is more serious.The two resistant biotypes AH-9 and AH-10 produced moderate cross-resistance to fenoxaprop-P-ethyl and Clethodim.The resistance of the above 7 resistant biotype DEN herbicides,Pinoxaden,produced a low level of cross-resistance.Three resistant biotypes,such as AH-3,AH-5 and AH-7,have moderate cross-resistance to mesosulfuron-methyl and Flucarbazone-Na,and low cross-resistance to Imazapic and Fispyribac-sodium.The AH-4 and AH-8 biotypes showed moderate cross-resistance to mesosulfuron-methyl,Imazapic and Flucarbazone-Na,and had low level of cross-resistance to Fispyribac-sodium.The AH-5 and AH-7 resistant biotypes showed different levels of multi-resistance to ACCase and ALS inhibitors,and belonged to more resistant multi-resistance populations.It is recommended to use Isoproturon equal to ACCase and ALS inhibitors in production.Different mechanisms of action are used to control the drug.(5)Mutations in the ACCase and ALS loci,and the metabolic mechanism mediated by the GSTs-metabolizing enzyme,may be the main resistance of the multi-resistant biotypes AH-5 and AH-7 to ACCase and ALS inhibitors.factor.This study clarified the resistance level,cross-resistance and multi-resistance of wheat-resistant Japanese maidens to fenoxaprop-P-ethyl and mesosulfuron-methyl.The results showed that the high level of resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl was mainly related to ACCase and ALS gene mutations,and may also be related to the metabolic mechanism mediated by GSTs-based metabolic enzymes.