Preliminary Study On The Effect Of ABA On Enamel During The Induction Of Plant By Penicillium Inactivated Mycelium Polypeptide

Controlling plant disease is a most important issue in agriculture.Compared with traditional chemicals,nature products has many advantages suchas broad spectrum,persistence and nonspecificity.It has a wide range of sources,environmentally sound and other advantages,in line with the needs of sustainable development of agriculture.Penicillium inactivated mycelium(dry mycelium of penicillium chrysogenum,DMP),as the residual by-product of penicillin extracted by pharmaceutical enterprises,is composed of fermentation residue,mycelium and metabolite.The early stageof the laboratory indicated that in the interaction between N.benthamiana and TMV,the polypeptide extract of Penicillium inactivated mycelium(PDMP)induced resistance,produced enamel deposition,and resisted the spread of the virus.Recently,it has been reported in the literature that abscisic acid(ABA)is involved in the accumulation of enamel,ABA can regulate the intensity and velocity of callose deposition.In order to explore the molecular regulation mechanism of sputum accumulation,the ABA inhibitor fluridone and ABA were sprayed with N.benthamiana,and then later subjected to PDMP,after induction treatment.The accumulation of enamel in the inoculated leaves was detected,the proliferation of TMV in different treatment groups was observed,the content of ABA in different treatment groups was determined,and the genes involved inCallose synthesis and the key genes involved in ABA formation were detected by real-time quantitative PCR.Toconclusion.there are the main results.(1)In the process of TMV infecting N.benthamiana,PDMP induced Callosedeposition at plasmodesmata may be one ofthe key factors to resist the spread of the virus.(2)After the exogenous spray inhibitor fluridone,the endogenous ABA content of the plant can be reduced.Similarly,external application of ABA also increases the ABA content in the plant.However,spraying ABA or fluridone alone did not affect the accumulation of enamel.(3)Fluridonecan promote the accumulation of enamel in plants after PDMP induction treatment.(4)The relative expression of scorpion-synthesis-related genes was significantly higher in the sputum synthesis-related genes than in the untreated control group,and the relative expression of ABA1 was decreased in the fluridone-treated group.(5)The leaves of TMV inoculated with fluridone before PDMP induction showed a significantly slower rate of fluorescence diffusion,indicating that the treatment effectively slowed the spread of the virus.In summary,the ABA inhibitor fluridone promoted the accumulation of enamel during the induction of TMV resistance by PDMP and enhanced disease resistance.