Design,Synthesis And Chemiluminescence Analysis Of Aggregation Induced Luminescent Cationic Surfactants

Chemiluminescence resonance energy transfer(CRET)is a powerful spectroscopy technique.Since the chemiluminescence donor in CRET does not require an external excitation light source and has no problems such as autofluorescence and photobleaching,it exhibits excellent performance in immunoassays,macromolecular analysis,small molecule detection,bioimaging,and therapeutic applications.Effectively improving the efficiency of CRET can not only improve its application performance,but also further expand its interdisciplinary applications.At present,the academic circles generally believe that the CRET process follows the F(?)rster resonance theory,that is,the CRET efficiency is inversely proportional to the sixth power of the CRET donoracceptor distance.However,the lack of specific experimental data to support this hypothesis limits the in-depth understanding of CRET.This thesis aims to design and synthesize a new type of cationic surfactant with aggregationinduced luminescence(AIE)characteristics,prepare cationic luminescent micelles with adjustable luminophore positions as CRET acceptors,and combine the luminol chemiluminescence system as CRET donors.In order to realize the controllable adjustment of the CRET donor-acceptor distance,explore and establish an analytical method for the quantitative relationship between the CRET efficiency and the donor-acceptor distance.The main research contents are as follows:1.By covalently attaching tetraphenylethylene(TPE)to the fourth,eighth and twelfth methyl groups adjacent to the cationic head group of dodecyltrimethylammonium bromide(C12TAB),design Three types of AIE cationic surfactants were synthesized and named C8-TPE-C4TAB,C4-TPEC8TAB and TPE-C12TAB.Characterized by nuclear magnetic and mass spectrometry,verified their molecular structure;through theoretical calculation and electrical conductivity measurement,obtained their hydrophilic and hydrophobic properties and critical micelle concentration(CMC)in water;through ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy,etc.Technology has studied their optical properties before and after the formation of micelles.The results show that C8-TPE-C4TAB,C4-TPEC8TAB and TPE-C12TAB have very similar hydrophilic and hydrophobic properties and optical properties,can form micelles at the same concentration in an aqueous solution,and produce strong fluorescence.2.Based on three luminescent micelles constructed by AIE-active cationic surfactants,the quantitative relationship between CRET efficiency and CRET donor-acceptor distance was experimentally determined.The morphology and particle size of the three luminescent micelles were characterized by transmission electron microscope(TEM)dynamic light scattering(DLS)and theoretical calculations.The luminol anion electrostatically adsorbed on the surface of the micelle was used as the CRET donor,and the TPE luminophores located at different positions inside the micelle were used as the CRET acceptor.The CRET efficiency under different luminol concentrations was measured by chemiluminescence spectroscopy.The results show that the CRET efficiency is inversely proportional to the sixth power of the CRET donor-acceptor distance,which is consistent with the F(?)rster resonance theory.