Multifunctional Nanocarriers Used In Image-guided Antitumor Therapy And Kinetic Study Of Enzyme Attached On Nanoparticles

In recent years,the application of nanomaterials in photothermal or photodynamic antitumor therapy has attracted extensive attention,but the single therapy has its own limitations.In this thesis,we designed and prepared a new type of multifunctional nano carrier,MSNR@Au-TPPS₄（Gd）,which was synthesized by using gadolinium ion chelated supramolecular photosensitizer TPPS₄ to modify the rod-shaped mesoporous silica coated gold shell.It can be applied to near-infrared fluorescence（NIRF）/multispectral optoacoustic tomography（MSOT）/computed tomography（CT）/magnetic resonance（MR）multimodal imaging guided photothermal（PTT）/photodynamic（PDT）synergistic antitumor therapy.Through the in vitro characterization of the nanomaterial and the experimental verification of its multimodal imaging guided photothermal and photodynamic synergistic therapy at the cellular and animal levels,we found that this multifunctional nanomaterial exhibited good imaging and tumor inhibition effects.On this basis,we continued to carry out the research on nanocarriers.Enzyme attached on nanoparticles have become another breakthrough point for anti-tumor medicine.In order to explore the change of enzyme catalytic activity in enzyme attached on nanoparticles,we selected thermophilic ethanol dehydrogenase（ht-ADH）with special discontinuous transition at 30℃as the research object.Different methods were used to obtain various ht-ADH attached on nanoparticles and their catalytic activities were measured.At present,the kinetic parameters of enzyme attached on nanoparticles are being measured.In conclusion,we successfully prepared multifunctional nanocarriers with a good combination of multimodal imaging and synergistic anti-tumor effects,enriching the diversity of multifunctional diagnostic and therapeutic nanocarriers.We also made a preliminary exploration of the kinetics of e enzyme attached on nanoparticles,which provided the principle and theoretical basis for the design and development of multifunctional nanomaterials in the future.