| Silk fibroin（SF）,a natural polymer material,has a broad research prospect in the field of biological medicine due to its adjustable biodegradability and excellent biological response in recent years.In this paper,methylcellulose/silk fibroin（MC/SF）composite hydrogels were prepared by sol-gel method,and the effects of changing the content of MC on the crystallization properties,structure,morphology,viscoelasticity and thermal stability of MC/SF composite hydrogels and the drug release properties of MC/SF composite hydrogels loaded with doxorubicin（DOX）were investigated.Sodium alginate/silk fibroin（SA/SF）composite hydrogels were prepared by physical crosslinking method.The effects of changing the content of SA on the crystallization properties,structure,morphology,thermal stability,viscoelasticity of SA/SF composite hydrogels and the drug release properties of DOX-loaded SA/SF composite hydrogels were investigated.Chitosan/silk fibroin（CS/SF）composite hydrogels were prepared by physical crosslinking method,and the effects of changing the content of CS on the crystallization properties,structure,morphology,thermal stability and viscoelasticity of CS/SF composite hydrogels and the drug release properties of DOX-loaded CS/SF composite hydrogels were investigated.The main contents are as follows:（1）MC/SF composite hydrogels were prepared by sol-gel method.The effects of changing the content of MC on the crystallization properties,morphology,thermal stability,structure and viscoelasticity of MC/SF composite hydrogels were investigated by XRD,SEM,TG and FT-IR tests.DOX was used as a drug model to prepare DOX loaded MC/SF composite hydrogels.The effects of changing MC content on drug release performance,LC and EE of the drug loaded composite hydrogels were investigated by UV-Vis spectrophotometer.The results showed that with the increase of MC content,the pore size of MC/SF composite hydrogels become smaller and takes the shape of honeycomb coal.The addition of MC improved the thermal stability of the composite hydrogels,and the thermal stability gradually increased with the increase of MC content.With the increase of MC content,the EE and LC of the DOX-loaded composite hydrogels also increased.The EE and LC of the MC（70%）/SF composite hydrogels reached 83.23±0.42%and 1.96±0.46%,respectively.While that of the pure SF hydrogel was only 69.18±0.95%and 1.62±0.51%.It can delay the release of DOX in vitro and prolong the release cycle of drug.（2）SA/SF composite hydrogels were prepared by physical crosslinking method.The effects of changing the content of SA on the crystallization properties,morphology,thermal stability,structure and viscoelasticity of SA/SF composite hydrogels were investigated.The DOX loaded SA/SF composite hydrogels were prepared to explore the effects of changing SA content on drug release performance,LC and EE.The conclusion showed that with the increase of SA addition,the pore size of SA/SF composite hydrogels become compact and the pore size become smaller.The thermal stability of composite hydrogels increased gradually with the increase of SA addition,indicating that the addition of SA improved the thermal stability of composite hydrogels.With the increase of SA addition,the EE and LC of the DOX-loaded composite hydrogels also increased,the release rate of drug DOX gradually decreased,and the release cycle of DOX extended.（3）CS/SF composite hydrogels were prepared by physical crosslinking method.The effects of changing the content of CS on the crystallization properties,morphology,structure,thermal stability and viscoelasticity of CS/SF composite hydrogels were investigated.The DOX loaded CS/SF composite hydrogels were prepared to explore the effects of changing CS content on drug release performance,LC and EE.The conclusion showed that with the increase of CS content,the number of CS/SF composite hydrogels pore increased in unit area.The addition of CS improved the thermal stability of the composite hydrogels,and the thermal stability gradually increased with the increase of CS content.The LC and EE of CS（30%）/SF composite hydrogels were 4.11±0.61%and82.10±0.39%,respectively.While the LC and EE of pure SF hydrogel was only3.74±0.35%and 74.89±0.57%.The cumulative drug release curve in vitro indicated that the DOX-loaded CS/SF composite hydrogels had a good sustained release effect. |
