Preparation Of Silk Fibroin Based Nanospheres By Self-assembly And Its Drug Release Properties

In recent years,silk fibroin(SF)materials have been widely used in biomedical fields due to their good biocompatibility and biodegradability.In this paper,the hydrophobic drug rifampicin(RIF)-loaded SF nanospheres were prepared by a simple and easy self-assembly method.The morphology,structure,crystallization properties and drug release properties of RIF-loaded SF nanospheres were investigated by changing the SF concentration and RIF addition.PLA-PEG/SF nanospheres were prepared by self-assembly method.The effects of the amount of PLA-PEG on the morphology,structure,crystallization and thermodynamic properties of PLA-PEG/SF nanospheres were studied.And the drug release properties of rhodamine B(RHB)-Ioaded PLA-PEG/SF nanospheres were studied by changing initial addition of PLA-PEG.Sodium alginate/silk fibroin(SA/SF)nanospheres were prepared by self-assemble method.The morphology,structure,crystallization properties and thermodynamic properties of SA/SF nanospheres were studied by changing initial addition of SA.And drug release properties of doxorubicin(DOX)-loaded SA/SF nanospheres were studied with increased of initial addition of SA.The main research contents of this paper are as follows:(1)Facile fabrication of Rifampicin-loaded silk fibroin nanospheres and its controlled drug release propertiesRIF-loaded SF nanospheres were prepared by self-assembly method.The morphology,conformation and crystallization properties of RIF-loaded silk fibroin nanospheres were investigated by SEM,XRD and FT-IR.The drug release properties of RIF-loaded SF nanospheres were studied by UV-Vis spectroscopy.The results showed that the average particle size distribution of RIF-loaded SF nanospheres could be effectively regulated at 123-245 nm by changing the concentration of silk fibroin and initial addition of drug rifampicin.By increasing the initial addition of rifampicin,the average particle size of RIF-loaded SF nanospheres decreased gradually.And the average particle size of RIF-loaded SF nanospheres gradually increased in higher SF concentration.The drug loading content and encapsulation efficiency of RIF-loaded SF nanospheres could also be regulated by changing SF concentration and RIF addition.With the addition of RIF and SF concentration increased,the drug loading content and encapsulation efficiency of RIF-loaded SF nanospheres were gradually increased.The LC and EE of RIF-loaded SF nanospheres were up to 3.13%and 37.61%respectively.With the increase of SF concentration and the addition of RIF,the drug release rate of RIF-loaded SF nanospheres can be effectively reduced and greatly prolong the cycle of drug release.(2)Preparation of polylactic acid-polyethylene glycol/silk fibroin nanospheres and its controlled drug release propertiesPLA-PEG/DMF solution was added to SF aqueous solution,and PLA-PEG/SF nanospheres were successfully prepared by self-assembly method.The effects of PLA-PEG amount on the morphology,structure,particle size,thermal stability and crystallization performance of PLA-PEG/SF nanospheres were investigated by changing the addition of PLA-PEG..RHB-loaded PLA-PEG/SF nanospheres were prepared by taking rhodamine B(RHB)as a hydrophilic cationic drug model.By changing the addition of PLA-PEG,the effects of the encapsulation efficiency,drug loading content and drug release properties of RHB-loaded PLA-PEG/SF nanospheres were investigated.The results showed that the average particle size distribution of RHB-loaded PLA-PEG/SF nanospheres could be effectively regulated at 388-487 nm by changing the initial addition of PLA-PEG.By increasing the initial addition of PLA-PEG,the average particle size of RHB-loaded PLA-PEG/SF nanospheres decreased gradually.Moreover,the addition of PLA-PEG improved the thermal stability of SF nanospheres,and the thermal stability of PLA-PEG/SF nanospheres increased with incresing the amount of PLA-PEG.The drug loading content and encapsulation efficiency of PLA-PEG/SF nanospheres could also be regulated by changing PLA-PEG addition.With the addition of PLA-PEG increased,LC and EE of PLA-PEG/SF were gradually increased.The drug loading content and encapsulation efficiency of RHB-loaded PLA-PEG/SF nanospheres were up to 7.38%and 85.02%respecti,vely.With the increase of the addition of PLA-PEG,the drug release rate of RHB-loaded PLA-PEG/SF nanospheres can be effectively reduced and greatly prolong the cycle of drug release.(3)Manufacture of sodium alginate/silk fibroin nanospheres and its controlled drug release propertiesThe good stability of sodium alginate(SA)was introduced into the silk fibroin material,and sodium alginate/silk fibroin(SA/SF)nanospheres were prepared by self-assembly method.The morphology,structure,particle size distribution,solution stability of SA/SF nanospheres were investigated by adjusting the addition of SA.DOX-loaded SA/SF nanospheres were prepared using DOX as hydrophilic agent by self-assembly method.The results showed that the average particle size distribution of DOX-loaded SA/SF nanospheres could be effectively regulated at 502-667 nm by changing the initial addition of SA.By increasing the initial addition of SA,the average particle size of DOX-loaded SA/SF nanospheres decreased gradually.Moreover,the addition of SA improved the thermal stability of SF nanospheres,and the thermal stability of SA/SF nanospheres increased with increasing the amount of SA.The drug loading content and encapsulation efficiency of SA/SF nanospheres could also be regulated by changing SA addition.With the addition of SA increased,the LC and EE of SA/SF nanospheres were gradually increased.The drug loading content and encapsulation efficiency of DOX-loaded SA/SF nanospheres were up to 5.34%and 84.83%respectively.With the increase of the addition of SA,the drug release rate can be effectively reduced and greatly prolong the cycle of drug release.