Synthesis And Performance Of Several "on-off" Hydrazine Hydrate Fluorescent Probes

Small molecules are widely distributed in the environment and living organisms.Many small molecules participate in daily biological metabolism and many chemical reactions.However,if small molecules exceed a certain limit,they are also harmful.Therefore,for the qualitative and quantitative determination of small molecules and specific imaging study it is very important.Hydrazine hydrate in which a wide range of industrial applications as a small molecule due to its high toxicity,highly carcinogenic and got people special attention.Traditional means for detecting the current hydrazine hydrate mainly composed of small molecules following method:electrochemical process,the color matching method,spectrophotometry,titration,high performance liquid chromatography.Since the probe was detected by simple fluorescence hydrazine hydrate operation error is relatively small,a higher sensitivity in recent years has been the concern of many researchers.It is worth mentioning that the fluorescent probe compared to those of conventional detection methods,can be achieved in vivo traditional means of identification was not possible to fine fluorescence imaging.Currently,hydrazine hydrate on recognition mechanism may be substantially divided into two aspects,（1）based on the identification of hydrazine hydrate reactive fluorescent probes,（2）identifying a material type based on hydrazine hydrate fluorescent probes.Wherein the fluorescent probe is widely applied to identify the principle reactive hydrazine hydrate,hydrazine-type synthetic material difficulties faced with respect to the fluorescent probe,fluorescence probe includes a synthetic reactive due to a relatively simple,readily available raw materials and so on.This paper mainly chalcone derivatives,isophorone derivatives,salicyl aldehyde derivative as the fluorescent matrix,hydrazine hydrate by known mature recognition mechanism designed rationally synthesized three different recognition sites.the reactive hydrazine hydrate fluorescent probe,colorimetric,fluorescence and fluorescence spectra colorimetric analysis of Hela cell fluorescence confocal imaging methods,studied the performance of these three specifically identified using fluorescent probes hydrazine hydrate naked eye.Wherein specific system consists of three parts:The first part is an introduction about the hydrazine hydrate fluorescent probes,in which we ordered introduces the characteristics and specific applications of hydrazine hydrate,hydrazine hydrate and illustrates the enormous harm to the environment and human body;introduces the related fluorescent probe of design principles and several identification mechanisms.We reasonably designed fluorescent probes related to three kinds of hydrazine hydrate,and some simple introduction of these fluorescent probes of hydrazine hydrate.Based on the second portion of hydrazine hydrate is capable of specifically acetyl and cut withα-βunsaturated ketone cyclization reaction occurs,designed fluorescent probe T-1.In order to achieve complete water solubility,we encapsulated T-1 in m PEG-DSPE polymer to design nano fluorescent probe Nano T-1.The detection limit is 6.92×10^-7mol/L.The internal biological avoid interference associated autofluorescence by confocal microscopy imaging,the successful application of hydrazine hydrate in Hela cell fluorescence imaging,and finally confirmed by Nano T-1 on Hela cells can achieve specific recognition of hydrazine hydrate.In the third part,we are based on the ability of hydrazine hydrate to specifically cut the acetyl and dinitrile groups.The structure of the probe molecule T-2 was analyzed and the related fluorescence properties of the fluorescent molecule T-2 were analyzed subsequently.It can be seen from the results that the probe molecule T-2 has good performance in naked-eye recognition,fluorescence recognition,and anti-interference analysis of hydrazine hydrate.It can be applied to the colorimetric detection of hydrazine hydrate in the environment with a detection limit of 8.37×10^-8mol/L.In the fourth part,a recent new ring-opening mechanism for identifying hydrazine hydrate was used.Finally,we synthesized a reactive hydrazine hydrate fluorescent probe molecule T-3 through a simple reaction design.As with the previous two parts,the same related structure analysis and fluorescence test were carried out.From the results,it can be seen that the probe molecule T-3 has good performance in naked-eye recognition,fluorescence recognition,and anti-interference analysis of hydrazine hydrate.