The Construction And Applications Of Hydrazine Fluorescent Probes Based On Different Mechanisms

In recent years,with the increasing awareness of environmental protection,the detection of industrial emissions has become more and more strict.To respond positively to environment protection policies at home and abroad,the development of a convenient and effective contaminant has become an important subject for many research groups.This year,the advancement and development of science and technology have brought the gospel to experimental study,especially the emergence of fluorescen detection has become an important research method.At the same time,many small molecule fluorescent probes have been reported.At present,small molecule fluorescent probes are generally based on the detection of common metal ions and chemicals.However,the toxicity of hydrazine has not been discovered for a long time,and the related fluorescent probes have rarely been reported.Hydrazine,as a common reactive group,the excessive emissions by the widespread use is inevitably harmful to the environment and human beings.Therefore,it is necessary to design and synthesize a hydrazine probe with high sensitivity,high specificity and convenient detection to explore and monitor excessive hydrazine.In response to this problem,this thesis carried out research on hydrazine probes,designed and synthesized three hydrazine probes based on different mechanisms,and explored the detection of hydrazine by probes through UV fluorescence and other related tests.The specific work is as follows:1.The coumarin derivative was selected as a fluorophore,and a "ture-on" probe 1 was designed and synthesized.The hydrazine can cleaved the acetoxy group of the probe to release fluorescence,thereby achieving the detection of hydrazine.Probe 1 is sensitive and fast to hydrazine,and its selectivity to hydrazine is higher than other amines,the detection limit was as low as 2.98 nM.In addition,the probe was successfully applied to Hela cells imaging.2.The coumarin derivative was selected as the fluorophore,and the ratio fluorescent probe 2 was designed and synthesized.One of the amino group of hydrazine can react with the carbonyl group at the 4-position,and the other amino group can replace the hydrogen at the 3-position to form a stable ring.Through the detections,the spectral performance of probe 2 was improved compared to probe 1,and the detection mechanism was first discovered.Probe 2 is also available for HeLa cells imaging.3.The 1,8-naphthalimide was selected as the fluorophore,and the colorimetric ratio type fluorescent probe 3 was designed and synthesized.This work showed that hydrazine as a nucleophile can break the ester bonds to extend conjugate resulting in cyan fluorescence shifting orange fluorescence.Probe 3 further improved optical properties compared to the above two probes.In practical applications,it has not only been successfully applied to cells imaging,but also successfully used for the detection of hydrazine gas.