Research On The Construction And Application Of Fluorescent Probes Based On Tetraphenyl Ethylene And Triarylboron

The rapid development of modern industry inevitably causes serious pollution to the air,soil and water resources,including the fresh water resources and groundwater resources in most areas.As the top of the food chain,human beings accumulate excessive metal and anion in soil and water with the enrichment of elements,which cause various diseases and even life-threatening,such as common copper poisoning,mercury poisoning and chronic fluorosis.However,people only pay attention to metal ions and ignore the pollution caused by anions,but their harm can’t be underestimated.The traditional methods for the detection of fluorine include ion selective electrode and ion chromatography.The methods for detecting metal ions are atomic absorption spectrometry and inductively coupled plasma mass spectrometry.These methods require the operation of professionals and expensive instruments and equipment,and require a lot of pretreatment preparation.In general,the concentration of pollutants in the detection system is low,the composition of components is complex,and the sensitivity and selectivity of detection methods are required.Fluorescence analysis has many advantages,such as high sensitivity,high selectivity,fast real-time detection,simple operation,etc.,and has been widely used in the detection and analysis of bioactive molecules,anions and metal ions.Based on the fluorescent chromophore of Tetraphenylethylene(TPE)with aggregation induced effect(AIE),two kinds of fluorescent probes of tetraphenylethylene derivatives were designed and synthesized.The function of the probes for the identification and detection of fluoride and metal ions was studied,and the detection limits and selectivity were further explored.The main contents of this paper are as follows:1.Based on the strong Lewis acid of triaryl boron and the steric resistance effect under specific structure,it can be used to identify Lewis bases with small volume.A set of tetraphenylethylene asymmetric isomer fluorescent probes modified with TPA and dimethyboron functional groups were designed and synthesized,2-6(E)and 2-7(Z).In anhydrous tetrahydrofuran solution,TBAF was added to probe 2-6(E)and 2-7(Z),and the fluorescence of probe molecule was enhanced.According to the linear relationship,the detection limit of 2-6(E)to fluoride ion was 2.96 μM.The detection limit of 2-7(Z)for fluoride ion is 2.78 μM.The response time is 10 s.2.Tris(2-methylpyridine)amine(TPA)is a group with strong chelating effect on metal ions.A group of symmetrical isomer fluorescent probes 3-2(E)and 3-3(Z)with TPE and TPA structures were designed and synthesized.In tetrahydrofuran solution containing 10%water,the fluorescence of the probe is completely quenched.The detection limit of trans structure 3-2(E)for copper ion is 9.02 μM.The detection limit of CIS structure 3-3(Z)is1.17 μM.It shows that the TPA group of CIS structure has a good ability to "capture" copper ions,and the response time is 10 s.