Process Control And Application Of Cellulase Production By Trichoderma Reesei In Fed-batch Batch Fermentation

Cellulase is the third largest category of industrial enzymes in China,but because the enzyme production strength is low and the cost of the refining process is high,resulting in its not only low yield and very expensive,in the refining process of bio-butanol,cellulase is 25%of the entire process cost,is a bottleneck link that restricts the mass production of bio-butanol push and wide use.China’s 14 th Five-Year Plan proposes to improve green low-carbon development,achieve carbon neutrality,strengthen the use of renewable resources,mainly straw agricultural waste energy,feed,fertilizer and new materials,in these for the reuse of biomass resources,are largely dependent on the role of cellulase,and cellulase in the medical,food engineering,paper,detergent and other industries also play a certain role.The role of cellulase in medical,food engineering,paper,detergent and other industries.Therefore,exploring cost-effective cellulase production methods is of great significance for the utilization of biomass renewable resources,saving the use of fossil energy,reduction of carbon emissions and mitigation of global warming.In this paper,we optimized the reverse reaction conditions of transglycosylation reaction,and constructed a cost-effective preparation method of soluble Trichoderma reesei enzyme inducer.And after exploring the three conditions of initial glucose concentration,temperature and enzyme addition,it was found that under the optimal conditions of glucose concentration of 850 g/L,temperature of 55°C and addition of 25 CBU/m L per gram of glucose The highest concentration of sophorose in the inductant was 165 g/L.2% concentration of inducer had the best effect on promoting the enzyme production of Trichoderma reesei.T.reesei Rut C30 was used as the experimental strain to optimize its fermentation enzyme production,and the enzyme activity of the filter paper reached 37 FPU/m L under optimal conditions,and it was verified that this method is also applicable to the fermentation enzyme production of genetically engineered modified bacterium T.reesei PB3.The enzyme solution produced by the original strain T.reesei Rut C30 and the genetically engineered bacterium T.reesei PB3 was used for the enzymatic digestion of dilute sulfuric acid pretreated straw,and the enzymatic system was compounded with Novozymes’ commercial enzyme Ctec II to evaluate the effect of the cellulase produced in the laboratory.and the enzymatic solution was applied to butanol fermentation.The hydrolysis effect of the enzyme solution produced by fermentation with the uncompounded engineered strain T.reesei PB3 was found to be superior to that of the commercial enzyme.