Construction Of Fluorescent Probes Based On SiQDs And CQDs And Their Application In Drug Detection

Quantum dots,as a novel kind of fluorescent nanomaterials with unique structures,physical and chemical properties,are widely used in medicine,biology,physics,chemistry and other fields.At present,various quantum dots have been reported including precious metal clusters,silicon quantum dots(SiQDs)and carbon quantum dots(CQDs).Among them,fluorescent probes based on SiQDs and CQDs hold great potentialin biomolecular imaging and analytical sensing.The good optical stability,tunable fluorescence emission and low preparation cost of CQDs provide advantages to further extend their applications in biosensors,fluorescence imaging and other fields.Although a large number of CQDs with excellent properties and simple synthesis process have been reported,the research on multi-mode detection of CQDs is still in its infancy and needs to be further improved.In addition,SiQDs have attracted extensive attention of researchers because of their several fascinating properties such as low toxicity,good water solubility,resistance to photobleaching and high stability.A large number of literatures about the preparation and application of SiQDs have been reported.Many fluorescent probes based on SiQDs are capable of detecting metal ions,small biological molecules,but there are few reports on drug analysis.Therefore,it is of great significance to develop SiQDs with simple preparation procedures in detecting drugs.For the above reason,this paper carried out the following research work:1、The application of water-soluble carbon dots with both fluorescent and colorimetric modes in detection of hypochloriteIn this work,water-soluble carbon dots(G-CQDs)were synthesized through hydrothermal method,which can detect Cl O~-in both fluorescent and colorimetric modes.After adding different concentrations of Cl O~-to G-CQDs,the color of the solution changes from light yellow to orange under sunlight and obvious fluorescence quenching can be observed under UV light.This method can detect Cl O~-sensitively and accurately with a linear rang of 0-300μM and a detection limit of 38 n M.In addition,G-CQDs can be used to detect Cl O~-in real samples with good recovery,and also can be used in cell experiment.2、A novel rt-SiQDs prepared at room temperature were used to detect L-DopaIn this work,rt-SiQDs with good water solubility,high stability,excellent biocompatibility was synthesized at room temperature,which had fluorescence enhancement response to L-Dopa.Therefore,L-Dopa can be quantitatively determined by rt-SiQDs.MTT results showed that rt-SiQDs had low cytotoxicity and had potential in biological detection.3、One-step synthesis of b-SiQDs and its application in the identification of naproxen enantiomersIn this work,water-soluble b-SiQDs were synthesized in one step at room temperature,R-NAP can enhance the fluorescence of b-SiQDs,but S-NAP has no response.So this sensor can be used toidentify naproxen(NAP)enantiomers in 20 s and quantitatively determin R-NAP(S-NAP has no influence).