| Hydrogen peroxide is an important disinfectant in production and life as well as the product of a variety of enzymatic reactions.The efficient detection of hydrogen peroxide is of great significance in the research fields of food,biology and clinic.Therefore,many methods have been developed for the analysis of hydrogen peroxide,including fluorescence method,chromatographic electrochemical method,etc.Among these methods,electrochemical method is considered to be the most effective because of its simplicity and high sensitivity.By further modification of electrochemical hydrogen peroxide sensor,glucose sensor can be prepared,which is of great significance for a large number of diabetic patients.In this paper,based on Prussian blue（PB）modified the main structure of porous gold,the hydrogen peroxide sensor was developed.At the same time,the glucose biosensor was further prepared by adsorption-crosslinking solid-enzyme method,and the flexible technology was explored to explore feasible solutions for flexible medical devices.Specific research contents are as follows:（1）The gold and silver alloy films（with an atomic ratio of 35:65）were de-alloyed by free corrosion dealloying to obtain the porous gold.Besides,the duration of dealloying was studied,and the corresponding pore sizes were obtained under different dealloying time.（2）The hydrogen peroxide sensor was prepared by electrochemically depositing Prussian blue onto a porous gold nanoelectrode.The modified electrode was tested by timing current method and cyclic voltammetry.The results showed that the sensor had good electrochemical performance and the electrode significantly enhanced the direct electron transfer from PB nanocrystalline to NPG film.Compared with the previous PB modified electrode,the sensor prepared this time has the widest linear range,0.001-17 mM,and has a high sensitivity of 708μA·cm-2·mM-1.（3）To investigate and optimize the electrochemical deposition time,a key factor in hydrogen peroxide sensor.Through structural and electrochemical characterization,we observed significant differences in sensitivity,linear range and stability of the sensors at different deposition times.The critical deposition time td=120 s was determined.Under this deposition condition,the PB lattice enveloped the ligaments of NPG and formed a solid PB adhesive layer with a thickness of 300 nm above the NPG layer.（4）On the basis of hydrogen peroxide sensor,glucose sensor was further prepared.Adopted adsorption and crosslinking method of combining the two kinds of solid enzyme way,by physical adsorption of chitosan with glutaraldehyde crosslinking,glucose oxidase layer is closely connected with the Prussian blue layer,then coated with a certain concentration of nafion solution to prevent loss at the same time improve the selectivity of sensor for glucose enzyme layer,the preparation of sensor linear range 100-1400μM with sensitivity of 10.2μA·mM-1·cm-2.（5）A flexible film based on Prussian blue modified electrode was prepared by a flexible process.On the hard substrate with a spin coating way to shake a layer of PMMA,then to shake a layer of PI after PMMA curing,then growth of chromium in E/B way as binder,and a layer of gold,magnetron sputtering（lithography-development-etching）by the semiconductor process will not need to remove,after transfer to PDMS,a flexible Prussian blue film can be obtained by reactive ion etching and electrochemical deposition. |
PDF Full Text Request