Preparation Of Ly(N-acetylaniline)-Prussian Blue Hybrid Composite Film And Application To Hydrogen Peroxide Sensing

The detection of hydrogen peroxide (H2O2) is an important topic for environmental control and clinical diagnosis due to it is not only a product of industry but also a risk factor for some diseases. Prussian Blue (PB), known as an artificial peroxidase, has been extensively used for H2O2detection and been a fundamental material for possible mass production of biosensors, due to its excellent catalysis for H2O2reduction at low potentials compared to noble metal, and a relatively cheap and stable electro-catalyst compared to enzyme. However, the primary problem of using PB as catalyst for detection of H2O2is the poor operational stability at neutral and alkaline solutions.Recently, hybrid film, which based on integration of PB and conducting polymers, has both properties of the individual components with the synergistic effect. Conducting polymers has their unique electronic and mechanical properties, as well as biocompatibility. However, as pH of the media increased above4, the polymer would lose its electrochemical activity, the applications in bioelectrochemistry are limited in neutral aqueous solutions. By now, large kinds of conducting polymer/PB composite films, specially composites of PB and PANI have been wildly studied. However, conducting hybrid composites using PB and polyaniline derivations are seldom studied. On the other hand, many reports were focused on the avenue of step by step for deposition, but the procedure of co-deposition have rarely been reported on this field. The main aspects were as follows:1A novel conductive polymer poly(N-acetylaniline)-Prussian blue (PNAANI-PB) hybrid film was prepared by one step co-electrodeposition in electroplating bath simultaneously containing low concentration of N-acetylaniline (10mM) and FeCl3-K3[Fe(CN)6]4. The structure and electro-activity of the PNAANI-PB hybrid film were evaluated. The effects of potential scanning cycles and the concentration of N-acetylaniline on the formation of the hybrid composite film were investigated. The hybrid film demonstrated rapid response, good reproducibility, long-term stability and free of interference towards reduction of H2O2. A glucose biosensor was further fabricated by immobilization of glucose oxidase on the hybrid film and applied to detect glucose in human serum samples. 2A novel conductive polymer PB/PNAANI-PB hybrid film was prepared by two steps. Firstly, the pure PB was electrodeposited on the GCE to form the negative charges of the surface of the electrode. Secondly, the PNAANI-PB hybrid film was co-electrodeposited on the pure PB film, by one step in electroplating bath simultaneously containing high concentration of N-acetylaniline(30mM) and FeCl3-K3[Fe(CN)6]4. The structure and electro-activity of the PB/PNAANI-PB hybrid film were investigated. The effects of potential scanning cycles in the both two steps were also investigated. The hybrid film demonstrated high current sensitivity, good reproducibility, and long-term stability towards reduction of H2O2.3In this work, a new methods for "in situ" chemically synthesized PB was proposed, and a novel route for fabrication PNAANI-PB hybrid composites is proposed by the spontaneous redox reaction in the FeCl3-K3[Fe(CN)6] and the N-acetylaniline solution. PB was easily synthesized from the FeCl3-K.3[Fe(CN)6] solution by using N-acetylaniline as reducer and chemical oxidation polymerization of N-acetylaniline simultaneously. With the existence of electrostatic interaction, the PNAANI-PB hybrid composites show synergistic augmentation of the response current for H2O2detection. The PNAANI-PB hybrid composites films exhibits high sensitivity, rapid response, good reproducibility, and long-term stability.