| currently,diabetes（a metabolic disease characterized by high blood glucose levels）has been found to have greatly caused various deaths worldwide.In order to prevent and diagnose diabetes,regular monitoring of blood glucose levels is always emphasized as a means of disease assessment and management.In the last few years,most researchers have devoted themselves to developing enzyme-based electrochemical sensors,especially glucose oxidase sensors As a low-cost and low-toxic nanomaterial,zinc oxide（ZnO）has the advantages of simple structure,easy synthesis at low temperatures,high crystallinity,good optical properties and good electrical properties.Because of its diverse nanostructure morphology and high specific surface area,it has a huge capacity to accommodate enzymes,thereby improving the overall sensing performance of the enzyme sensor.Because of this,ZnO has shown great advantages in biomolecular detection of enzyme sensors.In this paper,ZnO nanorods were prepared by a two-step method and characterized by X-ray diffractometer（XRD）and scanning electron microscope（SEM）.The influence of precursor solution concentration and hydrothermal temperature on the polar growth of ZnO nanorods was studied.And using electrochemical workstation to study the electrochemical performance of ZnO nanorod-based biosensor,the main research content and results are as follows:The ZnO seed layer solution was prepared with zinc acetate dihydrate as the solute,absolute ethanol as the solvent,and ethanolamine as the complexing agent.The ZnO seed layer was prepared by spin-coating method,and the influence of different concentration,spin-coating number and sintering temperature on the polar growth of ZnO seed layer was tested and analyzed by XRD and SEM.It is found that high-concentration ZnO seed layer solution is not suitable for growing seed layer.The best experimental conditions for ZnO seed layer are:concentration 0.50 mol/L,spin-coating number 5 layers,sintering temperature 330℃.Using zinc nitrate hexahydrate and hexamethylenetetramine as solutes and deionized water as solvent,a precursor solution of ZnO nanorods was prepared.The setting time is 6 h,ZnO nanorods were prepared by hydrothermal method,and the influence of different concentrations and temperatures on the polar growth of ZnO nanorods was tested and analyzed by XRD and SEM.The best experimental conditions for ZnO nanorods were determined:Time 6 h,concentration 0.05 mol/L,temperature 85 ℃.ZnO nanorods are used as matrix materials,and glucose oxidase is attached to the ZnO nanorods through physical adsorption to prepare ZnO nanorod-based glucose biosensors.The electrochemical performance of the sensor was tested by an electrochemical workstation.Get the following results:Cyclic voltammetry was used to measure the current response of the electrode in PBS and glucose solution respectively,and it was concluded that glucose oxidase was adsorbed on the electrode,and the oxidation-reduction reaction occurred under the action of the enzyme.Using cyclic voltammetry to measure the current response of glucose solutions with different concentrations,it is concluded that the peak current of the oxidation peak increases with the increase of the concentration of the glucose solution,and the enzyme electrode can quantitatively detect glucose.By measuring the cyclic voltammetry curve at different scan rates,the peak current of the oxidation peak at different scan rates is linearly related to the scan rate.The linear equation is y=0.33x-0.48,the correlation coefficient R=0.99,and the electrochemical process is surface controlled process.The calibration curve of current response and glucose concentration is y=11.60x+2.59 by chronoamperometry.The correlation coefficient is R2=0.99,the linear detection range of the electrode is 0-4.65 mM,and the sensitivity is 23.21μAmM-1cm-2.By adding glucose,UA,DA,AA and fructose solution to the PBS solution for anti-interference ability test,it is concluded that the electrode has a strong anti-interference ability. |
PDF Full Text Request