| The technology of drug active ingredient screening is the technology of screening active ingredients which have synergistic effect on the treatment of diseases from complex samples.Among them,the active ingredients can achieve the purpose of treating intractable diseases by interfering with the network of biological cells.Due to the complexity of screening,the existing screening technology for active ingredients of pharmaceuticals cannot meet the requirements of fast,accurate,simple,convenient and high reproducibility for screening active ingredients.In order to overcome the above shortcomings,improve the reliability of active ingredient screening and speed up the analysis and detection,this paper develops a miniature multi-chamber electrophoresis active ingredient screening system based on multi-chamber electrophoresis separation technology.The system is mainly composed of five parts:condensation structure,circulation structure,power supply structure,separation structure and human-computer interaction interface.IAP15W413 AS MCU is used as the control core of the system to realize the integration of electrophoresis tank and electrophoresis instrument.First of all,according to the principle of multi compartment electrophoresis,a model of electrophoresis cell was designed to separate the target complex and small molecular components.At the same time,the system realizes the function of electrolyte circulation flow through two peristaltic pumps and one air pump,and condenses the temperature of the electrophoresis tank through a high-power condensing plate and a radiator,and realizes the integration of the electrophoresis tank and the electrophoresis instrument.Secondly,this paper designs the condenser control circuit,electrophoresis voltage control circuit,electrophoresis current detection circuit,temperature detection circuit,air pump drive circuit,peristaltic pump drive circuit,MCU interface circuit,power supply circuit and other hardware circuits.At the same time,the control and connection between each module of the system are programmed and debugged,and the hardware and software design of the system is completed.Finally,the performance test of the commissioned experimental prototype was carried out.Through the experiment,it is verified that the flow rate error of the peristaltic pump is less than 2% in the flow rate range of 0 to 30 ml/min.At the same time,the condensation temperature of the electrophoresis tank is controlled between 10℃ to 20℃,and the temperature error after stabilization is less than ±0.2℃,which meets the performance index of the system design.Through the screening experiment of HSA standard samples,recording and analyzing the experimental data,it is verified that the mobility of the target substance can reach more than 70% in just 10 minutes of electrophoresis time,which compared with the 50% mobility result of traditional active ingredient screening technology,this system greatly improves the efficiency and reproducibility of active ingredient screening. |
