Surface Plasmon Coupling Enhanced Electrochemiluminescence Strategy For Biosensing Application

Electrochemiluminescence（ECL）is a process in which luminophores on the surface of an electrode are triggered by electrochemical methods to generate an excited state and produce light emission.The surface plasmon coupling enhanced electrochemiluminescence sensor based on surface plasmon coupling effect can enhance the ECL intensity of the luminophore,which greatly improves the sensitivity of the ECL analysis method.This article introduced three different plasmon materials and elaborated the influence of plasmon materials on the surface plasmon coupling enhanced electrochemiluminescence of carbon nitride quantum dots.Meanwhile,the surface plasmon coupling enhanced electrochemiluminescence sensor of carbon nitride quantum dots were constructed for detecting tumor markers.The article includes four parts:In the first chapter,the definition,characteristics and types of ECL are briefly reviewed.Then,the mechanism and advantages of quantum dots in ECL,the application of carbon nitride quantum dots in ECL sensing and the research progress of surface-enhanced ECL are described in detail.Finally,the research significance and main content of this paper are briefly introduced.In the second chapter,a yolk-shell composite material with ferroferric oxide as the magnetic core and gold shell as the outer shell was synthesized.The existence of a hollow cavity in the structure changed the medium of the surrounding environment of the gold shell,and generated a whispering gallery mode,which caused a surface plasmon coupling effect.Therefore,we constructed a surface plasmon coupling enhanced ECL sensor sensing platform based on yolk-shell nanomaterials,and accurately detected the K-RAS gene.In the third chapter,concave gold cubes of different sizes were prepared and tunable plasmon absorption properties were realized.Then the influence of the size,morphology and spectral overlap of the plasmon material on the ECL intensity of quantum dots and the surface plasmon coupling enhanced electrochemiluminescence sensing mode was discussed.After that,we combined concave gold cubes with toehold-mediated strand displacement technology to construct an ECL sensor and quantitatively analyzed the BRAF gene.In the 4^th chapter,we fabricated plasmon materials the gold dimers using the DNA rigid chain structure.The internal distance of the gold dimers was precisely regulated by the length of the DNA strand.We discussed in detail the relationship between the internal distance of the gold dimers and the surface plasmon coupling effect.Finally,we designed a surface plasmon coupling enhanced ECL sensing method to quantitatively detect the BRCA1 gene.