Surface Plasmon Coupling-amplified Electrochemiluminescence Strategy Of Boron Nitride Quantum Dots For Biosensing Research

Electrochemiluminescence（ECL）method integrates the advantages of the electrochemical method and the spectroscopic method,which has a unique advantage in analytical performance.The coupling effect induced by plasmon resonance on the surface of noble metal nanostructures can be used to amplify the ECL signal.The surface plasmon coupling-enhanced ECL is also called SEECL.Boron nitride quantum dots（BN QDs）are a new kind of nanoparticles derived from two-dimensional sheet materials.BN QDs have good optical characteristics,high thermal conductivity,good chemical stability,dispersibility and biocompatibility.As a new type of ECL probe,BN QDs have a wide band gap,which can be adjusted by heteroatom doping to improve their photoelectric properties.This article mainly studies the construction of surface plasmon-coupled signal amplification ECL DNA sensors based on BN QDs and metal nanomaterials,which is mainly divided into the following four parts:In the chapter 1,we introduced the development of QDs-based ECL sensor,the synthesis methods and properties of BN QDs,and the research of the surface plasmon coupling ECL.And we briefly discussed the research significance and main content of this paper.In the chapter 2,we prepared BN QDs as ECL probe via microwave-assisted hydrothermal synthesis.The distance between BN QDs and gold nanoparticles（Au NPs）was regulated via hairpin DNA.As a result,a novel kind of resonance energy transfer/surface plasmon coupled ECL sensor was constructed for the quantitative detection to STEC gene.In the chapter 3,we used thiourea and L-cysteine as two precursors to synthesize two kinds of sulfur-doped BN QDs（S-BN QDs）with different ECL properties.Based on the difference ECL peaks of two S-BN QDs,we designed a dual-wavelength surface plasmon coupling ratio sensor with the target catalytic hairpin assembly（CHA）technology to achieve detection of BRAF gene.In the chapter 4,we used S-BN QDs as ECL probes,and molybdenum disulfide（MoS₂）nanosheets as surface plasma material.We discussed in detail the effect of distance on the surface plasmon coupling effect using DNA strands of different lengths.A surface plasmon-coupled ECL sensor has been designed based on the hybrid chain reaction（HCR）to quantitatively detect HCV gene.