Study On The Separation And Analysis Of Active Components Of Traditional Chinese Medicine By High Performance Capillary Electrophoresis

The high capillary electrophoresis method has the advantages of high separation performance and low reagents consumption,and is widely applied the field of separation and analysis.In this paper,we established three new capillary electrophoresis methods for the separation and analysis of the main active ingredients in medicines based on the summary of additives used in capillary electrophoresis and development of coupled with mass spectrometry technology,it has practical value and provides new choice for the quality control of Chinese herbal medicines and their preparations,the main research details are as follows:1.The new micellar capillary electrophoresis method was established for simultaneous separation of three chromones including cimicifuga,cimicifugain and 5-O-methylsammioside.The three investigated compounds can reach baseline separation and quantitative determination within 15 minutes in running electrolyte solution containing24%（v/v）methanol,8 mmol/L SDS,1.6%（v/v）isopropanol,Na₂B₄O₇（20 mmol/L）-Na OH（28 mmol/L）buffer（p H 9.73）among the conditions of the separation voltage of 25k V,capillary temperature of 25℃,detector wavelength of 254 nm and injection time of5 s（3447.38 Pa）.The concentration of three investigated compounds have a good linear relationship with the peak area in the range of 5.0～100.0μg/m L.The method was used to analysis and determination of the three active ingredients in Chinese herbal medicines Saposhnikoviae Radix,Fangfengtongsheng pills and Fenghanganmao granules.The results showed that the recovery ranged of each compound was between 95.7%～104.3%and the relative standard deviation was below 3.0%.2.The new capillary electrophoresis method was established for simultaneous separation of four alkaloids including rhynchophylline,isorhynchophylline,dehydrorhynchophylline and isodehydrorhynchophylline.The four investigated compounds can reach baseline separation and quantitative determination within 10minutes in running electrolyte solution containing 30 mmol/L S-β-CD,Na H₂PO₄（39mmol/L）-Na₂HPO₄ buffer（p H 9.42）among the conditions of the separation voltage of 18k V,capillary temperature of 25℃,detector wavelength of 250 nm and injection time of5 s（3447.38 Pa）.The linear ranges of four investigated compounds were 5.0～120.0μg/m L,5.0～120.0μg/m L,5.0～140.0μg/m L and 5.0～100.0μg/m L,respectively.The method was used to analysis and determination of the four active ingredients in Uncaria pieces,Baoji pills and Xiaoerqixing Tea.The results showed that the recovery ranged of each compound was between 95.1%～103.5%and the relative standard deviation was below4.0%.3.A simple and efficient capillary electrophoresis analysis method was established for simultaneous separation of five flavonoids including icariin,icariin II,homidine A,homidine B and homidine C with the conditions of detailed optimized electrophoresis determination.The final optimized conditions were containing 25%（v/v）methanol,0.4mmol/L SDS and Na₂B₄O₇（20 mmol/L）-Na OH（24 mmol/L）buffer（p H 9.62）as the running electrolyte solution and the separation voltage of 25 k V,capillary temperature of25℃,detector wavelength of 270 nm,and injection of 3 s（3447.38 Pa）.The five investigated compounds can reach baseline separation within 20 minutes after the conditions.The linear ranges of icariin,icariin II,homidine A,homidine B and homidine C were 5.0～120.0μg/m L,5.0～100.0μg/m L,5.0～140.0μg/m L,5.0～100.0μg/m L and5.0～160.0μg/m L,respectively.The method was used to analysis and determination of the five active ingredients in Epimedium and its preparations among the recovery ranged between 96.3%～104.8% and the relative standard deviation of below 4.0%.