| As the most abundant transition metal element in human body,iron is involved in numerous physiological processes like oxygen transportation,electron transfer and immunological process,etc.Tracking the distribution and fluctuation of iron and monitoring its transfer and metabolism process have showed remarkable importance.In recent years,fluorescent probes were utilized in the detection of various biological species due to their high sensitivity/selectivity,high time-space resolution,and low tissue damage.Thus,fluorescent probes have gained more and more attention.Fe3+shows distinct quenching effect to many fluorophore due to its paramagnetic property,and thus most reported fluorescent probes displayed "turn-off" response to Fe3+,which means decrement of fluorescent emission and lack of bio-imaging application.Therefore "turn-on" or ratiometric probes become demanded and important in the detection of ironThis thesis contains two main parts:First,we will introduce the important biological functions of iron,some principles and classification of fluorescent probe analysis as well as introduction of several typical reported Fe3+probesFour 1,8-naphthalimide-rhodamine B derivated FRET-based ratiometric fluorescent probes will be discussed in the second part focusing on the disadvantages of previously designed probes.The four probes were divided into two groups R1,R2 and R3,R4 according to the different connecting site of 1,8-naphthalimide moiety.Spectroscopic study reveals all four probes show fine sensitivity and selectivity to Fe3+ over other physiological metal cations,Probes R3/R4 show better FRET efficiency over R1/R2,with which the relationship between the FRET efficiency and their structure can be discussed. |
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