The Effect Of Ginsenoside Rg3 On The Viability Of Human Glioma Cells And Its Rapid Quantitative Identification

Ginsenoside Rg3(G-Rg3)is an anti-cancer active substance extracted from ginseng.It can not only be used in the adjuvant treatment of cancer to enhance the effects of radiotherapy and chemotherapy,but also because of its ability to cultivate vitality and replenish vital energy.Health benefits such as blood,anti-fatigue,and immunity enhancement are often used as the main components of certain functional foods.In recent years,the incidence of glioma has increased year by year,and G-Rg3 has become one of the current research hotspots in cancer diseases because of its significant anti-cancer activity.In addition,although G-Rg3 has a broad market prospect,there are many news reports that there are a large number of products on the market advertised as G-Rg3,but the G-Rg3 content is very small,which will not only make consumers pay high The cost may also cause some cancer patients with asymmetric information to delay treatment and seriously endanger their lives.The current commonly used detection methods require a large amount of samples and the operation is cumbersome and complicated,which increases the cost of detection.Therefore,in actual production supervision,there is an urgent need for a simple,accurate,rapid,continuous and minute quantitative identification method.This topic selects G-Rg3 as the research object,uses human glial cells(HEB)and brain glioma cells(U87)to establish a cell experimental model,and combines CCK-8 cytotoxicology experiments to study the effect of G-Rg3 on the two influence of biological activity,combined with optical microscope technology and cell staining technology,further studied their changes in biological morphology and quantity.Then use the attenuated total reflection based terahertz time domain spectroscopy(THZ TD-ATR)technology to establish a set of dielectric extraction methods for cells in the THz frequency band,calculate the dielectric characteristics of the cells,and systematically analyze different cells,Dielectric differences between different numbers of cells and between different biological activities of cells.Finally,based on THZ TD-ATR technology,a set of micro-nano flow channel chips for trace solution detection was developed,and applied to different concentrations of trace G-Rg3 solutions for quantitative identification research,establishing a predictive model,and determining the best Identify the conditions.The results of the study are as follows:(1)G-Rg3 has an inhibitory effect on HEB cells and U87 cells,and its survival rate is negatively correlated with the concentration and duration of action.However,the survival rate of U87 cells after G-Rg3 has a significantly greater decline than HEB cell.(2)As the concentration of G-Rg3 increases,the number and morphology of cells change.G-Rg3 has no significant effect on HEB cells but significant effect on U87 cells.Among them,the HEB cytoskeleton after G-Rg3 treatment is complete,the nucleus is full,and the number of cells proliferates normally.After G-Rg3treatment,the U87 cytoskeleton has abnormal characteristics,the nucleus begins to become smaller and missing,and the cell shrinks and rounds.,The overall condition becomes worse,the number of cells becomes less,and it is difficult to find the corresponding cells in the field of view.(3)There are differences in the dielectric characteristics between different types and different concentrations of the same type of cells(this article is mainly reflected in the absorption coefficient).The absorption coefficient of U87 cells shows a negative correlation with cell concentration,while the absorption coefficient of HEB cells at different concentrations There was a slight positive correlation trend.Under the same conditions,the absorption coefficient of U87 cells was lower than that of HEB cells.(4)The THZ TD-ATR technology can reflect the effect of G-Rg3 on cell biological activity to a certain extent.After normalizing the spectral results,the U87 cell results show a similar change trend with the cell survival rate.The HEB cell results It is opposite to the cell survival rate.(5)The use of micro-nano flow channel chip can realize the continuous and rapid detection of trace G-Rg3solution.The established quantitative identification model has the best effect at 1.0 THz and1.2 THz,and the unary linear regression model at 1.0 THz is y=-17.87x+250.74,the R~2 of the model is 0.94,the RMSE is 1.64,the one-variable linear regression model at 1.2 THz is y=-24.04x+284.89,the R~2 of the model is 0.90,and the RMSE is 2.81,which are obtained at these two frequencies The prediction result recovery rate is between 77.5%-125%,the recovery rate is high,and the accuracy rate is good.