Preparation Of A Molecularly Imprinted Photonic Crystal Based On Fusaric Acid And Its Application In The Detection Of Banana Fusarium Wilt

Banana fusarium wilt is a soil-borne disease caused by Fusarium oxysporum f.sp.cubense(Foc),which has caused great damage to the banana planting industry.Due to the strong vitality of Foc to survive in the soil,once the pathogen invades a banana planting area,it is very likely that bananas cannot be planted in that area.At present,there is no effective method to prevent and control banana fusarium wilt.The most important means to prevent and control banana fusarium wilt in agricultural production is to control the spread and invasion of pathogens.Among them,accurate detection of banana fusarium wilt is very essential.The current main detection methods for banana Fusarium wilt mainly rely on gene sequencing which is accurate but costly and time-consuming.If the rapid detection of banana wilt pathogen can be achieved,it will be of great significance for the effective prevention and control of banana wilt.We used the fusarium wilt pathogen to specifically produce Fusaric acid(FA)in the metabolic process,combined with the molecularly imprinted photonic crystal(MIPC),designed and synthesized a molecularly imprinted photonic crystal based on fusaric acid(MIPC-FA),through the qualitative detection of FA to achieve rapid qualitative detection of banana Fusarium wilt and further explored the the degrees of response to FA under different cross-linking degrees.Based on this,Foc-containing medium and banana plants infected by Foc were tested in a simulated environment.The main work of this paper includes the following two parts:(1)Monodisperse SiO₂ microspheres with uniform particle size were synthesized and SiO₂ photonic crystals were prepared on this basis.The synthesis process and the preparation process of photonic crystals were optimized,and the prepared SiO₂ photonic crystals and molecularly imprinted photonics crystals were systematically characterized the microscopic morphology and optical properties of the crystal;(2)The specificity of the molecularly imprinted photonic crystal film based on fusaric acid(MIPC-FA)to FA and the degrees of response of MIPC-FA with different crosslinking degrees to fusarium acid were characterized,and its performance was optimized.The research results show that MIPC-FA has specific responsive to FA,and its responsive is positively correlated with FA concentration in a wide concentration range(10^-7-10^-4 g/m L).Combined with HPLC detection and verification,it is confirmed that MIPC-FA can effectively detect FA in Foc-containing medium and banana plants infected by Foc.