Synthesis Of CuS Nanoparticles By BSA Template For The Detection And Therapy Of Tumor Cells

Bovine serum albumin(BSA)has been widely used as a multifunctional nanocarrier because of its biocompatibility,non-toxicity and non-immunogenicity,easy production and low cost,good stability in vivo,and easy group modification.Near infrared(NIR)photoresponsive nanoparticles have excellent tissue penetration ability for cancer treatment.They can also promote controlled release of anti-cancer drugs.They have a promising application on capture and release of circulating tumor cells(CTCs).In this paper,BSA was used as the template to synthesize photothermal CuS@BSA nanocomposites for the detection and therapy of tumor cells by their different surface modifications.The main contents of this thesis were showed as follows:(1)First,CuS nanoparticles were synthesized in situ using BSA templates to prepare CuS@BSA nanocomposites with NIR photothermal conversion ability.Then,Rhodamine110(Rho110)was covalentally coupled with CuS@BSA to construct CuS@BSARho110 nanocomposites.It was found that CuS@BSA-Rho110 could be effectively endocytotic by breast cancer cells(MCF-7),and accumulated in the multicelluar spheroids composed with parental MCF-7 cells,and successfully enriched specifically in mitochondria.But CuS@BSA had no mitochondrial targeting ability.At the same time,CuS@BSA-Rho110 showed enhanced effect of killing tumor cells under the same NIR laser irradiation.(2)CuS nanoparticles were synthesized in situ by BSA templates,and then CuS@BSA-Biotin nanocomposites were prepared by modifying Biotin on their surfaces.The microfluidic chip was designed and fabricated,using CuS@BSA-Biotin to capture and release of CTCs.The experimental results show that CuS@BSA-Biontin had a high affinity to capture MCF-7 cells with the efficiency of 45.1%.Moreover,the captured cells could be successfully released through NIR photothermal effect of CuS@BSABiontin,with the efficiency up to 81.1%.And ～78.6% of released cells kept good viability,and could be re-cultured and grew well,which facilitated the subsequent cellular analysis.