Differentiation Analysis Of Soy Hull Polysaccharides Migration In Simulated Intestinal Mucus Layer

Small particles and hydrocolloids which was digested by the intestine can diffuse freely from the intestinal cavity and be absorbed by intestinal cells.Due to the limitation of the viscosity of hydrocolloids,they may be blocked in the mucus layer during absorption.Therefore,the increase of viscosity of intestinal cavity usually affects the diffusion and hydrolysis of other hydrocolloids or mixed micelles.As a kind of functional hydrophilic colloid,the migration mechanism of soy hull polysaccharides（SHP）in the mucus layer is not clear.In this paper,microwave-assisted ammonium oxalate extraction of soy hull polysaccharides（MASP）and microwave-assisted sodium citrate extraction of soy hull polysaccharides（MSSP）was used as the object.The viscosity,migration and impact factors of MASP and MSSP were investigated by scanning electron microscope,rheometer,multiple light scattering and laser confocal microscope.The main conclusions are as following:1.The viscosity of MASP and MSSP decreased gradually with the increase of temperature.The viscosity of MASP and MSSP at p H 1.5 was greater than that at p H 8.0,and the viscosity of MSSP was greater than MASP at the same temperature and p H.The potential of interaction between MASP and intestinal mucus was 32.0 m V,while the potential of interaction between MSSP and intestinal mucus was 27.5 m V.The structure between MASP and intestinal mucus is loose,while the structure between MSSP and intestinal mucus is sticky.The migration amount of MASP was 13535.36 less than that of MSSP in the mucus layer,while the migration amount of MASP chyme was 20706.64 higher than that of MSSP in the mucus layer.2.Na⁺had little effect on the viscosity of polysaccharides,while Ca²⁺had a great effect on the viscosity of polysaccharides.The viscosity of MASP was greater than that of MSSP after Ca²⁺treatment.The potential of polysaccharides and intestinal mucus decreased after addition of Na⁺and Ca²⁺.Formation of stable complex structure of polysaccharides and mucins was inhibited by Na⁺and was promoted by Ca²⁺.The migration amount of MASP particles increased by 4888.67 after addition of Na⁺,and increased by 12874.17 after addition of Ca²⁺.The migration amount of MSSP particles increased by 2453.95 after addition of Na⁺and increased by 8166.3 after addition of Ca²⁺.The particle migration was inhibited after addition of Na⁺and Ca²⁺in chylous,and the inhibition effect of Na⁺is greater than Ca²⁺.3.The microstructure of polysaccharide and intestinal mucus containing Na⁺and Ca²⁺was modified by gut microbiota.The migration of MASP metabolite was increased by3158.96 after addition of Na⁺and gut microbiota,while decreased by 4076.58 after the addition of Ca²⁺and gut microbiota.The migration of MSSP metabolite was decreased by1504.05 after addition of Na⁺and gut microbiota,while increased by 2206.06 after the addition of Ca²⁺and gut microbiota.Na⁺significantly promoted the migration of acetic acid,propanoic acid and n-butyric acid produced by gut microbiota after catabolism of MASP,while Ca²⁺significantly inhibited the migration of isovaleric acid.Na⁺had no significant effect on the migration of short chain fatty acid（SCFA）produced by gut microbiota catabolism MSSP,while Ca²⁺had significant inhibitory effect on the migration of valeric acid.