Magnetic Resonance Imaging And Tumor Therapy Based On Mn²⁺-Doped Prussian Blue

Prussian blue nanoparticles are a type of inorganic nanomaterials which are formed by transition metal ions or lanthanide ions linked by cyanide bridging ligands.They haves adjustable chemical composition porous structure and good physical or chemical properties such as photothermal and magnetic properties.Prussian blue nanoparticles have been widely used in magnetic resonance imaging,photoacoustic imaging,ultrasound imaging,photothermal therapy,drug delivery nanocarriers and other medical imaging or therapy fields.In recent years,the research on Prussian blue nanoparticles has gradually become a hot spot in the field of nanomaterials.In this dissertation,Prussian blue doped with manganese ions was prepared by microemulsion method,then,applied in magnetic resonance imaging,photothermal therapy and chemical kinetic therapy.The main work of this paper is divided into the following two chapters:(1)The Prussian blue of manganese ion doping nanoparticles MnPB-NPs?(Mn2+:Fe2+=1:4)was synthesized by Triton X-100 microemulsion method.The inorganic nanoparticles have small particle size(?39 nm),good uniformity and dispersion,and the UV absorption peak locates around 713 nm.They exibit good photothermal stability and high photothermal conversion efficiency(44.32%).In vitro experimental,the MnPB-NPs II could be uptaken by HepG2 cells,and showed good effect of photothermal therapy(PTT)under the irradiation of 808 nm near-infrared laser,which is expected to be developed as a kind of good performance of field heat treatment.(2)The Prussian blue of manganese ion doping nanoparticles 30%MnPB(Mn2+:Fe2+=1:4)was synthesized by Triton X-100 microemulsion method.The inorganic nanoparticles have face-centered cubic structure,uniform particle size,and high absorption peak in the near infrared region.The r1 and r2 value reaches 0.78 mM-1·s-1 and 4.66 mM-1·s-1 on 7T magnetic resonance scanner,which realized T1-T2 dual-mode magnetic resonance imaging.30%MnPB can catalyze the decomposition of hydrogen peroxide to produce·OH,realizing the chemodynamic therapy(CDT).30%MnPB-PVP nanoparticles were prepared by coating the surface of 30%MnPB with polyvinylpyrrolidone(PVP).In vivo and in vitro experiments have shown that 30%MNPB-PVP has a good T1-T2 dual-mode magnetic resonance imaging effect,and can achieve significant tumor treatment effect through PTT/CDT synergistic therapy,which has great potential in the development and application of clinical diagnosis and treatment integrated probe.