Preparation And Function Of Microcrystalline Butyrylated Pea Starch

In this study,pea starch(PS)was used as raw material,and microcrystalline butyrylated pea starch(MBPS)was prepared by combining the existing preparation methods of microcrystalline starch and butyrate starch.The effects of acid hydrolysis and aqueous phase treatment on the structure,physicochemical properties and properties of PS were investigated.Fourier transform infrared spectroscopy,~1H-NMR,X-ray diffraction,ion chromatography,thermogravimetric analysis,scanning electron microscope and digestion in vitro were used to investigate the changes in structure and properties of MBPS compared with pea starch.The animal model of diabetes was established,and the fasting blood glucose,glucose tolerance,serum biochemical level,liver and nephropathy section of diabetic mice were measured to study the effect of MBPS on the basic symptoms of diabetes.Based on the metabonomics analysis of metabolites and microorganisms in mouse feces,the performance changes of MBPS compared with pea starch were further discussed,and the mechanism of lowering blood sugar and blood lipid by MBPS in diabetic mice was discussed from two aspects of diabetes indicators and metabolism of diabetic mice.MBPS was prepared from pea starch(PS)by acid hydrolysis and aqueous phase method.It was confirmed that the structure of PS changed and new groups appeared in pea starch by Fourier transform infrared spectroscopy and ~1H-NMR.The changes in structure and properties of MBPS compared with pea starch were studied by scanning electron microscope,X-ray diffraction,starch chain length distribution,thermogravimetric analysis and digestion in vitro.The results showed that the particle morphology and crystal structure of MBPS were the same as those of pea starch.Compared with pea starch,the relative crystallinity,proportion of long chain branches and content of resistant starch(RS)of MBPS were higher,and increases with the increase of degree of substitutione.Compared with pea starch,the ratio of thermal stability and short branch chain decreased.The C57BL/6J mouse model of type 2 diabetes was established by feeding with high fat diet and intraperitoneal injection of streptozotocin(STZ).During the experiment,diabetic mice were fed with MBPS(the sample replaced 20%of the starch of the original feed),and the effects of MBPS on fasting blood glucose,oral glucose tolerance,serum biochemical level,liver and kidney biopsy and other indicators of diabetic mice were studied.The results showed that feeding MBPS for 28 days could improve fasting blood glucose,serum biochemical level,oral glucose tolerance and liver and kidney injury in diabetic mice.With the increase of the degree of substitution of starch,its regulatory effect on diabetic mice also increased.It was proved that MBPS can improve diabetes.The intestinal microflora and metabolomics analysis method were adopted,and the flora and metabolism in feces of diabetic mice were detected by Illumina sequencing and LC-MS technology,and the effect of MBPS on metabolism of diabetic mice was systematically analyzed from the metabolic point of view.It could be seen that there is a great difference between diabetic mice in MBPS group and HFD group with different degrees of substitution.MBPS could also increase the proportion of beneficial bacteria and reduce the proportion of actinomycetes.MBPS can reduce the absorption of carbohydrates and long-chain saturated fatty acids,promote the excretion of2-ethylhexyl phthalate,and reduce the excretion of bile acids.MBPS4 and MBPS5starch with high degree of substitution could also regulate the abnormal metabolism of purine and pyrimidine and promote the pentose phosphate pathway.