Au-bi Bimetallic Nanomaterials Used In The Diagnosis And Treatment Of Tumors

In recent years,more and more attention has been paid to the diagnosis and treatment of tumors,and various new materials applied in this direction have been successively developed by researchers,especially multi-functional nanomaterials integrating diagnosis and treatment.As is known to all,metal nanomaterials have attracted the attention of scientific and technological workers because of their special physical and chemical properties:favorable stability,long fluorescence life,wide light absorption range and low biological toxicity.Among them,metal nanomaterials coated with thiols to form stable metal nuclear energy have the advantages of favorable stability,easy dispersion,small particle size and wide light absorption range,which can be applied to photothermal therapy(PTT)and photodynamic therapy(PDT)in the near infrared(NIR)region.Moreover,the structure of the metal core of the nanoparticle has important influence on its luminescence,catalysis and stability.However,until now,there have been few studies on the effects of bimetal on the production rate of reactive oxygen species(ROS)in tumor cells and their therapeutic efficiency.In this paper,bismuth was introduced into thiol-coated gold nanoparticles by one-step chemical reduction method to prepare ultra-small bimetallic core nanoparticles(Au₁Bi₁-SR).The introduction of Bi element can greatly improve the photothermal effect of nanoparticles,and the change trend of gold and bismuth bimetallic core nanoparticles thermal effect,ROS generation rate and tumor cell inhibition with the change of Au and Bi components was studied.Based on density functional theory of microstructure,the origin of photothermal of materials is deduced.The ROS production was detected by DCFH-DA probe,and the category of ROS was detected by electron paramagnetic resonance spectrometer(EPR).Finally,the therapeutic effects of the materials were detected by X-ray tomography(CT)imaging and photothermal imaging,and the therapeutic properties of Au₁Bi₁-SR were verified by cell experiments and in vivo mouse experiments.In addition,on the basis of Au₁Bi₁-SR this paper obtained Au₁Bi₁-SR@IR808 by surface modification of small molecule organic dye IR808,which effectively enhanced Au₁Bi₁-SR photothermal properties.After verification,Au₁Bi₁-SR@IR808 photothermal efficiency was improved by more than 6%compared with that of Au₁Bi₁-SR.Makes up for the insufficiency of its thermal efficiency,and eventually formed the shape stability,favorable dispersion,uniform particle size,high safety,low toxicity,and at the same time with CT,thermal imaging and photodynamic therapy,and photothermal therapy in the integration of new multifunctional nanomaterials of diagnosis and treatment of tumor,the material will further improve the level of diagnosis and treatment of tumor.